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Sulfate transporters involved in sulfate secretion in the kidney are localized in the renal proximal tubule II of the elephant fish (Callorhinchus milii)

Hasegawa, Kumi, Kato, Akira, Watanabe, Taro, Takagi, Wataru, Romero, Michael F., Bell, Justin D., Toop, Tes, Donald, John A. and Hyodo, Susumu 2016, Sulfate transporters involved in sulfate secretion in the kidney are localized in the renal proximal tubule II of the elephant fish (Callorhinchus milii), American journal of physiology: regulatory, integrative and comparative physiology, vol. 311, no. 1, pp. R66-R78, doi: 10.1152/ajpregu.00477.2015.

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Title Sulfate transporters involved in sulfate secretion in the kidney are localized in the renal proximal tubule II of the elephant fish (Callorhinchus milii)
Formatted title Sulfate transporters involved in sulfate secretion in the kidney are localized in the renal proximal tubule II of the elephant fish (Callorhinchus milii)
Author(s) Hasegawa, Kumi
Kato, Akira
Watanabe, Taro
Takagi, Wataru
Romero, Michael F.
Bell, Justin D.
Toop, Tes
Donald, John A.
Hyodo, Susumu
Journal name American journal of physiology: regulatory, integrative and comparative physiology
Volume number 311
Issue number 1
Start page R66
End page R78
Total pages 13
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2016-07
ISSN 0363-6119
1522-1490
Keyword(s) cartilaginous fish
kidney
sulfate transporter
secretion
elephant fish
Summary Most vertebrates, including cartilaginous fishes, maintain their plasma SO4 (2-) concentration ([SO4 (2-)]) within a narrow range of 0.2-1 mM. As seawater has a [SO4 (2-)] about 40 times higher than that of the plasma, SO4 (2-) excretion is the major role of kidneys in marine teleost fishes. It has been suggested that cartilaginous fishes also excrete excess SO4 (2-) via the kidney. However, little is known about the underlying mechanisms for SO4 (2-) transport in cartilaginous fish, largely due to the extraordinarily elaborate four-loop configuration of the nephron, which consists of at least 10 morphologically distinguishable segments. In the present study, we determined cDNA sequences from the kidney of holocephalan elephant fish (Callorhinchus milii) that encoded solute carrier family 26 member 1 (Slc26a1) and member 6 (Slc26a6), which are SO4 (2-) transporters that are expressed in mammalian and teleost kidneys. Elephant fish Slc26a1 (cmSlc26a1) and cmSlc26a6 mRNAs were coexpressed in the proximal II (PII) segment of the nephron, which comprises the second loop in the sinus zone. Functional analyses using Xenopus oocytes and the results of immunohistochemistry revealed that cmSlc26a1 is a basolaterally located electroneutral SO4 (2-) transporter, while cmSlc26a6 is an apically located, electrogenic Cl(-)/SO4 (2-) exchanger. In addition, we found that both cmSlc26a1 and cmSlc26a6 were abundantly expressed in the kidney of embryos; SO4 (2-) was concentrated in a bladder-like structure of elephant fish embryos. Our results demonstrated that the PII segment of the nephron contributes to the secretion of excess SO4 (2-) by the kidney of elephant fish. Possible mechanisms for SO4 (2-) secretion in the PII segment are discussed.
Language eng
DOI 10.1152/ajpregu.00477.2015
Field of Research 060603 Animal Physiology - Systems
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, American Physiological Society
Free to Read? No
Free to Read Start Date 2017-08-01
Persistent URL http://hdl.handle.net/10536/DRO/DU:30084328

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