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Alkaloid production and capacity for methyljasmonate induction by hairy roots of two species in Tribe Anthocercideae, family Solanaceae

Ryan, Suzanne M, Deboer, Kathleen D and Hamill, John D 2015, Alkaloid production and capacity for methyljasmonate induction by hairy roots of two species in Tribe Anthocercideae, family Solanaceae, Functional plant biology, vol. 42, no. 8, pp. 792-801, doi: 10.1071/FP15045.

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Title Alkaloid production and capacity for methyljasmonate induction by hairy roots of two species in Tribe Anthocercideae, family Solanaceae
Author(s) Ryan, Suzanne M
Deboer, Kathleen D
Hamill, John D
Journal name Functional plant biology
Volume number 42
Issue number 8
Start page 792
End page 801
Total pages 10
Publisher CSIRO Publishing
Place of publication Melbourne, Vic.
Publication date 2015-06-05
ISSN 1445-4408
1445-4416
Keyword(s) gene expression
hair roots
methyl-jasmonate induction
pyridine alkaloid
Solanaceae
tropane alkaloid
Summary In addition to producing medicinally important tropane alkaloids, some species in the mainly Australian Solanaceous tribe Anthocercideae, sister to genus Nicotiana, are known to also contain substantial levels of the pyridine alkaloids nicotine and nornicotine. Here, we demonstrate that axenic hairy root cultures of two tribe Anthocercideae species, Cyphanthera tasmanica Miers and Anthocercis ilicifolia ssp. ilicifolia Hook, contain considerable amounts of both nicotine and nornicotine (∼0.5-1% DW), together with lower levels of the tropane alkaloid hyoscyamine (<0.2% DW). Treatment of growing hairy roots of both species with micromolar levels of the wound stress hormone methyl-jasmonate (MeJa) led to significant increases (P<0.05) in pyridine alkaloid concentrations but not of hyoscyamine. Consistent with previous studies involving Nicotiana species, we also observed that transcript levels of key genes required for pyridine alkaloid synthesis increased in hairy roots of both Anthocercideae species following MeJa treatment. We hypothesise that wound-associated induction of pyridine alkaloid synthesis in extant species of tribe Anthocercideae and genus Nicotiana was a feature of common ancestral stock that existed before the separation of both lineages ∼15million years ago.
Language eng
DOI 10.1071/FP15045
Field of Research 060702 Plant Cell and Molecular Biology
0601 Biochemistry and Cell Biology
0602 Ecology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, CSIRO Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30084955

Document type: Journal Article
Collection: Faculty of Science, Engineering and Built Environment
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