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Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis

Phung, Sui Ching, Nai, Yi Heng, Macka, Mirek, Powell, Shane M, Guijt, Roseanne M and Breadmore, Michael C 2015, Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis, Analytical and bioanalytical chemistry, vol. 407, pp. 6995-7002, doi: 10.1007/s00216-015-8838-4.

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Title Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis
Author(s) Phung, Sui Ching
Nai, Yi Heng
Macka, Mirek
Powell, Shane M
Guijt, Roseanne M
Breadmore, Michael C
Journal name Analytical and bioanalytical chemistry
Volume number 407
Start page 6995
End page 7002
Total pages 8
Publisher Springer
Place of publication Berlin, Germany
Publication date 2015-09
ISSN 1618-2642
1618-2650
Keyword(s) capillary electrophoresis/electrophoresis
cell systems/single cell analysis
counter-pressure
capillary isotachophoresis
microbials
eletrokinetic injections
field amplified
Summary Counter-pressure was used to extend the duration of field-amplified sample injection in isotachophoresis (FASI-ITP) in order to improve the detection of bacterial cells. Using 0.51-μm negatively charged encapsulated fluorescent beads as a model, the counter-pressure, injection and separation voltages, and times were optimized. Using 6-min 8,963-Pa counter-pressure FASI-ITP injections at −12 kV followed by mobilization of the ITP band with continued injection at −6 kV, the limit of detection (LOD) for Escherichia coli was improved to 78 cells/mL, a factor of 4 when compared with FASI-ITP without counter-pressure.
Language eng
DOI 10.1007/s00216-015-8838-4
Field of Research 030199 Analytical Chemistry not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30084956

Document type: Journal Article
Collection: Faculty of Science, Engineering and Built Environment
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