In-silico optimisation of two-dimensional high performance liquid chromatography for the determination of Australian methamphetamine seizure samples Andrighetto, Luke, Burns, Niki K., Stevenson, Paul, Pearson, James, Henderson, Luke, Bowen, Christopher and Conlan, Xavier 2016, In-silico optimisation of two-dimensional high performance liquid chromatography for the determination of Australian methamphetamine seizure samples, Forensic science international, vol. 266, pp. 511-516, doi: 10.1016/j.forsciint.2016.07.016. Attached Files Name Description MIMEType Size Downloads Title In-silico optimisation of two-dimensional high performance liquid chromatography for the determination of Australian methamphetamine seizure samples Author(s) Andrighetto, Luke Burns, Niki K. Stevenson, Paul orcid.org/0000-0001-6780-6859 Pearson, James Henderson, Luke orcid.org/0000-0002-4244-2056 Bowen, Christopher Conlan, Xavier orcid.org/0000-0003-0829-0551 Journal name Forensic science international Volume number 266 Start page 511 End page 516 Total pages 6 Publisher Elsevier Place of publication Shannon, Ireland Publication date 2016-09 ISSN 0379-0738 Keyword(s) DryLab 2D-HPLC ephedrine methamphetamine Summary In-silico optimisation of a two-dimensional high performance liquid chromatography (2D-HPLC) separation protocol has been developed for the interogation of methamphetamine samples including model, real world seizure, and laboratory synthesised samples. The protocol used Drylab® software to rapidly identify the optimum separation conditions from a library of chromatography columns. The optimum separation space was provided by the Phenomonex Kinetex PFP column (first dimension) and an Agilent Poroshell 120 EC-C18 column (second dimension). To facilitate a rapid 2D-HPLC analysis the particle packed C18 column was replaced with a Phenomenex Onyx Monolithic C18 withought sacrificing separation performance. The Drylab® optimised and experimental separations matched very closely, highlighting the robust nature of HPLC simulations. The chemical information gained from an intermediate methamphetamine sample was significant and complimented that generated from a pure seizure sample. The influence of the two-dimensional separation on the analytical figures of merit was also investigated. The limits of detection for key analytes in the second dimension determined for methamphetamine (4.59 × 10-⁴ M), pseudoephedrine (4.03 × 10-4 M), caffeine (5.16 × 10-⁴ M), aspirin (9.32 × 10-4 M), paracetamol (5.93 × 10-4 M) and procaine (2.02 × 10-3 M). Language eng DOI 10.1016/j.forsciint.2016.07.016 Field of Research 030108 Separation Science Socio Economic Objective 0301 HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2016, Elsevier Ireland Persistent URL http://hdl.handle.net/10536/DRO/DU:30085171 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Centre for Biotechnology, Chemistry and Systems Biology Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 01 Aug 2016, 16:05:42 EST Mon, 01 Aug 2016, 16:06:15 EST Tue, 02 Aug 2016, 05:00:39 EST Tue, 02 Aug 2016, 08:11:25 EST Tue, 02 Aug 2016, 08:12:40 EST Sat, 20 Aug 2016, 06:10:40 EST Wed, 19 Oct 2016, 10:34:27 EST Wed, 19 Oct 2016, 10:39:44 EST Wed, 19 Oct 2016, 10:41:22 EST Wed, 11 Jan 2017, 09:47:54 EST Filtered Full Citation counts:   Cited 3 times in TR Web of Science Cited 3 times in Scopus Search Google Scholar Access Statistics: 265 Abstract Views, 4 File Downloads  -  Detailed Statistics Created: Mon, 01 Aug 2016, 16:06:15 EST

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