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Effects of down-regulating ornithine decarboxylase upon putrescine-associated metabolism and growth in Nicotiana tabacum L.

Dalton, Heidi L., Blomstedt, Cecilia K., Neale, Alan D., Gleadow, Ros, DeBoer, Kathleen D. and Hamill, John D. 2016, Effects of down-regulating ornithine decarboxylase upon putrescine-associated metabolism and growth in Nicotiana tabacum L., Journal of experimental botany, vol. 67, no. 11, pp. 3367-3381, doi: 10.1093/jxb/erw166.


Title Effects of down-regulating ornithine decarboxylase upon putrescine-associated metabolism and growth in Nicotiana tabacum L.
Formatted title Effects of down-regulating ornithine decarboxylase upon putrescine-associated metabolism and growth in Nicotiana tabacum L.
Author(s) Dalton, Heidi L.
Blomstedt, Cecilia K.
Neale, Alan D.
Gleadow, Ros
DeBoer, Kathleen D.
Hamill, John D.
Journal name Journal of experimental botany
Volume number 67
Issue number 11
Start page 3367
End page 3381
Total pages 15
Publisher Oxford University Press
Place of publication Oxford, England
Publication date 2016-05
ISSN 0022-0957
1460-2431
Keyword(s) alkaloid
gene expression
ODC
phenolamide
polyamine
putrescine
PMT
QPT
RNAi
Summary Transgenic plants of Nicotiana tabacum L. homozygous for an RNAi construct designed to silence ornithine decarboxylase (ODC) had significantly lower concentrations of nicotine and nornicotine, but significantly higher concentrations of anatabine, compared with vector-only controls. Silencing of ODC also led to significantly reduced concentrations of polyamines (putrescine, spermidine and spermine), tyramine and phenolamides (caffeoylputrescine and dicaffeoylspermidine) with concomitant increases in concentrations of amino acids ornithine, arginine, aspartate, glutamate and glutamine. Root transcript levels of S-adenosyl methionine decarboxylase, S-adenosyl methionine synthase and spermidine synthase (polyamine synthesis enzymes) were reduced compared with vector controls, whilst transcript levels of arginine decarboxylase (putrescine synthesis), putrescine methyltransferase (nicotine production) and multi-drug and toxic compound extrusion (alkaloid transport) proteins were elevated. In contrast, expression of two other key proteins required for alkaloid synthesis, quinolinic acid phosphoribosyltransferase (nicotinic acid production) and a PIP-family oxidoreductase (nicotinic acid condensation reactions), were diminished in roots of odc-RNAi plants relative to vector-only controls. Transcriptional and biochemical differences associated with polyamine and alkaloid metabolism were exacerbated in odc-RNAi plants in response to different forms of shoot damage. In general, apex removal had a greater effect than leaf wounding alone, with a combination of these injury treatments producing synergistic responses in some cases. Reduced expression of ODC appeared to have negative effects upon plant growth and vigour with some leaves of odc-RNAi lines being brittle and bleached compared with vector-only controls. Together, results of this study demonstrate that ornithine decarboxylase has important roles in facilitating both primary and secondary metabolism in Nicotiana.
Language eng
DOI 10.1093/jxb/erw166
Field of Research 060702 Plant Cell and Molecular Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID A19701779
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085240

Document type: Journal Article
Collection: Faculty of Science, Engineering and Built Environment
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Created: Thu, 04 Aug 2016, 12:08:14 EST

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