You are not logged in.
Openly accessible

Enhanced Ex Vivo expansion of human hematopoietic progenitors on native and spin coated acellular matrices prepared from bone marrow stromal cells

Wasnik, Samiksha, Kantipudi, Suma, Kirkland, Mark A. and Pande, Gopal 2016, Enhanced Ex Vivo expansion of human hematopoietic progenitors on native and spin coated acellular matrices prepared from bone marrow stromal cells, Stem cells international, vol. 2016, pp. 1-13, doi: 10.1155/2016/7231567.

Attached Files
Name Description MIMEType Size Downloads
wasnik-enhancedexvivo-2016.pdf Published version application/pdf 4.40MB 9

Title Enhanced Ex Vivo expansion of human hematopoietic progenitors on native and spin coated acellular matrices prepared from bone marrow stromal cells
Formatted title Enhanced Ex Vivo expansion of human hematopoietic progenitors on native and spin coated acellular matrices prepared from bone marrow stromal cells
Author(s) Wasnik, Samiksha
Kantipudi, Suma
Kirkland, Mark A.
Pande, Gopal
Journal name Stem cells international
Volume number 2016
Article ID 7231567
Start page 1
End page 13
Total pages 13
Publisher Hindawi Publishing Corporation
Place of publication New York, N.Y.
Publication date 2016
ISSN 1687-966X
1687-9678
Summary The extracellular microenvironment in bone marrow (BM) is known to regulate the growth and differentiation of hematopoietic stem and progenitor cells (HSPC). We have developed cell-free matrices from a BM stromal cell line (HS-5), which can be used as substrates either in native form or as tissue engineered coatings, for the enhanced ex vivo expansion of umbilical cord blood (UCB) derived HSPC. The physicochemical properties (surface roughness, thickness, and uniformity) of native and spin coated acellular matrices (ACM) were studied using scanning and atomic force microscopy (SEM and AFM). Lineage-specific expansion of HSPC, grown on these substrates, was evaluated by immunophenotypic (flow cytometry) and functional (colony forming) assays. Our results show that the most efficient expansion of lineage-specific HSPC occurred on spin coated ACM. Our method provides an improved protocol for ex vivo HSPC expansion and it offers a system to study the in vivo roles of specific molecules in the hematopoietic niche that influence HSPC expansion.
Language eng
DOI 10.1155/2016/7231567
Field of Research 060106 Cellular Interactions (incl Adhesion, Matrix, Cell Wall)
060103 Cell Development, Proliferation and Death
060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085292

Document type: Journal Article
Collections: Institute for Frontier Materials
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 46 Abstract Views, 9 File Downloads  -  Detailed Statistics
Created: Thu, 18 Aug 2016, 13:27:31 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.