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Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences

Lee, David, La Mura, Maurizio, Allnutt, Theo R. and Powell, Wayne 2009, Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences, BMC biotechnology, vol. 9, Article number : 7, pp. 1-7, doi: 10.1186/1472-6750-9-7.

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Title Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences
Author(s) Lee, David
La Mura, Maurizio
Allnutt, Theo R.
Powell, Wayne
Journal name BMC biotechnology
Volume number 9
Season Article number : 7
Start page 1
End page 7
Total pages 7
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2009-02-02
ISSN 1472-6750
Keyword(s) base sequence
brassica napus
DNA Primers
DNA, plant
molecular sequence data
plants, genetically modified
polymerase chain reaction
sensitivity and specificity
sequence analysis, DNA
transgenes
Summary BACKGROUND: The most common method of GMO detection is based upon the amplification of GMO-specific DNA amplicons using the polymerase chain reaction (PCR). Here we have applied the loop-mediated isothermal amplification (LAMP) method to amplify GMO-related DNA sequences, 'internal' commonly-used motifs for controlling transgene expression and event-specific (plant-transgene) junctions.

RESULTS: We have tested the specificity and sensitivity of the technique for use in GMO studies. Results show that detection of 0.01% GMO in equivalent background DNA was possible and dilutions of template suggest that detection from single copies of the template may be possible using LAMP.

CONCLUSION: This work shows that GMO detection can be carried out using LAMP for routine screening as well as for specific events detection. Moreover, the sensitivity and ability to amplify targets, even with a high background of DNA, here demonstrated, highlights the advantages of this isothermal amplification when applied for GMO detection.
Language eng
DOI 10.1186/1472-6750-9-7
Field of Research 060499 Genetics not elsewhere classified
Socio Economic Objective 920499 Public Health (excl. Specific Population Health) not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085305

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.