Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis Torey, Angeline, Vijayarathna, Soundararajan, Jothy, Subramanion L., Gothai, Sivapragasam, Chen, Yeng, Latha, Lachimanan Yoga, Kanwar, Jagat R., Dharmaraj, Saravanan and Sasidharan, Sreenivasan 2016, Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis, Journal of traditional and complementary medicine, vol. 6, no. 1, pp. 97-104, doi: 10.1016/j.jtcme.2014.11.017. Attached Files Name Description MIMEType Size Downloads kanwar-explorationof-2016.pdf Published version application/pdf 1.48MB 111 Title Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis Formatted title Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis Author(s) Torey, Angeline Vijayarathna, Soundararajan Jothy, Subramanion L. Gothai, Sivapragasam Chen, Yeng Latha, Lachimanan Yoga Kanwar, Jagat R. orcid.org/0000-0003-3728-9568 Dharmaraj, Saravanan Sasidharan, Sreenivasan Journal name Journal of traditional and complementary medicine Volume number 6 Issue number 1 Start page 97 End page 104 Total pages 8 Publisher Center for Food and Biomolecules, National Taiwan University Place of publication Taipei, Taiwan Publication date 2016-01 ISSN 2225-4110 Keyword(s) anticandidal mechanism cell wall protein glucosidase morphology potassium leakage proteinase Summary Candida albicans has become resistant to the commercially available, toxic, and expensive anti-Candida agents that are on the market. These factors force the search for new antifungal agents from natural resources. Cassia spectabilis had been traditionally employed by healers for many generations. The possible mechanisms of the C. spectabilis leaf extract were determined by potassium leakage study and the effect of the extract on the constituents of the cell wall and enzymes as well as the morphological changes on C. albicans cells were studied along with cytotoxicity assays. The cytotoxicity result indicated that the extract is nontoxic as was clearly substantiated by a half maximal inhibitory concentration (IC50) value of 59.10 μg/mL. The treated cells (C. spectabilis extract) demonstrated potassium leakage of 1039 parts per million (ppm) compared to Amphotericin B (AmpB)-treated cells with a released potassium value of 1115 ppm. The effects of the extract on the cell wall proteins illustrated that there were three major types of variations in the expression of treated cell wall proteins: the presence of new proteins, the absence of proteins, and the amount of expressed protein. The activities of two enzymes, α-glucosidase and proteinase, were determined to be significantly high, thereby not fully coinciding with the properties of the antifungal reaction triggered by C. spectabilis. The morphology of C. albicans cells treated with the C. spectabilis extract showed that the cells had abnormalities and were damaged or detached within the microcolonies. Our study verifies C. spectabilis leaf extract as an effective anti-C. albicans agent. Language eng DOI 10.1016/j.jtcme.2014.11.017 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2014, Center for Food and Biomolecules, National Taiwan University Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30085326 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 11 Aug 2016, 05:00:25 EST Thu, 11 Aug 2016, 06:09:19 EST Thu, 18 Aug 2016, 13:29:35 EST Thu, 18 Aug 2016, 13:36:43 EST Thu, 18 Aug 2016, 13:46:07 EST Wed, 05 Oct 2016, 09:00:55 EST Wed, 05 Oct 2016, 09:06:46 EST Thu, 06 Oct 2016, 08:32:40 EST Mon, 10 Apr 2017, 10:47:59 EST Fri, 05 May 2017, 10:14:11 EST Fri, 07 Sep 2018, 12:32:36 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 6 times in Scopus Search Google Scholar Access Statistics: 392 Abstract Views, 113 File Downloads  -  Detailed Statistics Created: Thu, 18 Aug 2016, 13:29:34 EST

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