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Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis

Torey, Angeline, Vijayarathna, Soundararajan, Jothy, Subramanion L., Gothai, Sivapragasam, Chen, Yeng, Latha, Lachimanan Yoga, Kanwar, Jagat R., Dharmaraj, Saravanan and Sasidharan, Sreenivasan 2016, Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis, Journal of traditional and complementary medicine, vol. 6, no. 1, pp. 97-104, doi: 10.1016/j.jtcme.2014.11.017.

Title Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis
Formatted title Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis
Author(s) Torey, Angeline
Vijayarathna, Soundararajan
Jothy, Subramanion L.
Gothai, Sivapragasam
Chen, Yeng
Latha, Lachimanan Yoga
Kanwar, Jagat R.
Dharmaraj, Saravanan
Sasidharan, Sreenivasan
Journal name Journal of traditional and complementary medicine
Volume number 6
Issue number 1
Start page 97
End page 104
Total pages 8
Publisher Center for Food and Biomolecules, National Taiwan University
Place of publication Taipei, Taiwan
Publication date 2016-01
ISSN 2225-4110
Keyword(s) anticandidal mechanism
cell wall protein
potassium leakage
Summary Candida albicans has become resistant to the commercially available, toxic, and expensive anti-Candida agents that are on the market. These factors force the search for new antifungal agents from natural resources. Cassia spectabilis had been traditionally employed by healers for many generations. The possible mechanisms of the C. spectabilis leaf extract were determined by potassium leakage study and the effect of the extract on the constituents of the cell wall and enzymes as well as the morphological changes on C. albicans cells were studied along with cytotoxicity assays. The cytotoxicity result indicated that the extract is nontoxic as was clearly substantiated by a half maximal inhibitory concentration (IC50) value of 59.10 μg/mL. The treated cells (C. spectabilis extract) demonstrated potassium leakage of 1039 parts per million (ppm) compared to Amphotericin B (AmpB)-treated cells with a released potassium value of 1115 ppm. The effects of the extract on the cell wall proteins illustrated that there were three major types of variations in the expression of treated cell wall proteins: the presence of new proteins, the absence of proteins, and the amount of expressed protein. The activities of two enzymes, α-glucosidase and proteinase, were determined to be significantly high, thereby not fully coinciding with the properties of the antifungal reaction triggered by C. spectabilis. The morphology of C. albicans cells treated with the C. spectabilis extract showed that the cells had abnormalities and were damaged or detached within the microcolonies. Our study verifies C. spectabilis leaf extract as an effective anti-C. albicans agent.
Language eng
DOI 10.1016/j.jtcme.2014.11.017
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Center for Food and Biomolecules, National Taiwan University
Free to Read? Yes
Use Rights Creative Commons Attribution Non-Commercial No-Derivatives licence
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Document type: Journal Article
Collection: School of Medicine
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