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4-Hydroxy-N-propyl-1,8-naphthalimide esters: new fluorescence-based assay for analysing lipase and esterase activity

Nalder, Tim, Ashton, Trent, Pfeffer, Frederick, Marshall, Susan N. and Barrow, Colin 2016, 4-Hydroxy-N-propyl-1,8-naphthalimide esters: new fluorescence-based assay for analysing lipase and esterase activity, Biochimie, vol. 128-129, pp. 127-132, doi: 10.1016/j.biochi.2016.07.016.

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Title 4-Hydroxy-N-propyl-1,8-naphthalimide esters: new fluorescence-based assay for analysing lipase and esterase activity
Author(s) Nalder, TimORCID iD for Nalder, Tim orcid.org/0000-0001-8748-7429
Ashton, TrentORCID iD for Ashton, Trent orcid.org/0000-0002-6707-6064
Pfeffer, FrederickORCID iD for Pfeffer, Frederick orcid.org/0000-0002-5441-6437
Marshall, Susan N.
Barrow, ColinORCID iD for Barrow, Colin orcid.org/0000-0002-2153-7267
Journal name Biochimie
Volume number 128-129
Start page 127
End page 132
Total pages 6
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-09
ISSN 1638-6183
1638-6183
Keyword(s) carboxylester hydrolase
enzyme assay
fluorescence
lipase
1,8-Naphthalimide
Summary Research using 1,8-naphthalimide derivatives has expanded rapidly in recent years owing to their cell-permeable nature, ability to target certain cellular locations and fluorescent properties. Here we describe the synthesis of three new esters of 4-hydroxy-N-propyl-1,8-naphthalimide (NAP) and the development of a simple and sensitive assay protocol to measure the activity of carboxylester hydrolases. The NAP fluorophore was esterified with short (butyrate), medium (octanoate) and long (palmitate) chain fatty acids. The esters were spectroscopically characterised and their properties investigated for their suitability as assay substrates. The esters were found to be relatively stable under the conditions of the assay and levels of spontaneous hydrolysis were negligible. Non-specific hydrolysis by proteins such as bovine serum albumin was also minimal. A simple and rapid assay methodology was developed and used to analyse a range of commercially available enzymes that included enzymes defined as lipases, esterases and phospholipases. Clear differences were observed between the enzyme classes with respect to the hydrolysis of the various chain length esters, with lipases preferentially hydrolysing the medium chain ester, whereas esterases reacted more favourably with the short ester. The assay was found to be highly sensitive with the fluorophore detectable to the low nM range. These esters provide alternate substrates from established coumarin-based fluorophores, possessing distinctly different excitation (447 nm) and emission (555 nm) optima. Absorbing at 440-450 nm also offers the flexibility of analysis by UV-visible spectrophotometry. This represents the first instance of a naphthalimide-derived compound being used to analyse these enzymes.
Language eng
DOI 10.1016/j.biochi.2016.07.016
Field of Research 060101 Analytical Biochemistry
0601 Biochemistry And Cell Biology
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM).
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085331

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