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Isolation and detection of microRNA from the egg of chickens

Wade, Ben, Cummins, Michelle, Keyburn, Anthony and Crowley, Tamsyn 2016, Isolation and detection of microRNA from the egg of chickens, BMC research notes, vol. 9, Article number: 283, pp. 1-6, doi: 10.1186/s13104-016-2084-5.

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Title Isolation and detection of microRNA from the egg of chickens
Author(s) Wade, Ben
Cummins, Michelle
Keyburn, Anthony
Crowley, Tamsyn
Journal name BMC research notes
Volume number 9
Season Article number: 283
Start page 1
End page 6
Total pages 6
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2016-05-23
ISSN 1756-0500
Keyword(s) Albumen
Chicken
Egg
Extraction
Yolk
microRNA
Summary BACKGROUND: The egg is a vital part of the chicken developmental process and an important protein source for humans. Despite the chicken egg being a subject of intense research little attention has been given to the role of microRNAs within the egg.

FINDINGS: We report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. We also report the detection via real-time PCR of a number of miRNAs from both of these biological fluids.

CONCLUSIONS: These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg. This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and also how this impacts the nutritional value of eggs for human consumption.
Language eng
DOI 10.1186/s13104-016-2084-5
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085464

Document type: Journal Article
Collections: School of Medicine
Open Access Collection
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Created: Tue, 11 Oct 2016, 07:51:04 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.