Rhizophagus irregularis as an elicitor of rosmarinic acid and antioxidant production by transformed roots of Ocimum basilicum in an in vitro co-culture system

Srivastava, Shivani, Conlan, Xavier A., Cahill, David M. and Adholeya, Alok 2016, Rhizophagus irregularis as an elicitor of rosmarinic acid and antioxidant production by transformed roots of Ocimum basilicum in an in vitro co-culture system, Mycorrhiza, vol. 26, no. 8, pp. 919-930, doi: 10.1007/s00572-016-0721-4.

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Title Rhizophagus irregularis as an elicitor of rosmarinic acid and antioxidant production by transformed roots of Ocimum basilicum in an in vitro co-culture system
Author(s) Srivastava, Shivani
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Cahill, David M.ORCID iD for Cahill, David M. orcid.org/0000-0002-2556-0528
Adholeya, Alok
Journal name Mycorrhiza
Volume number 26
Issue number 8
Start page 919
End page 930
Total pages 12
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-11
ISSN 1432-1890
Keyword(s) antioxidants
elicitor
transformed roots
in vitro
ocimum basilicum
rhizophagus irregularis
rosmarinic acid
Summary Arbuscular mycorrhiza is a symbiotic association formed between plant roots and soil borne fungi that alter and at times improve the production of secondary metabolites. Detailed information is available on mycorrhizal development and its influence on plants grown under various edapho-climatic conditions, however, very little is known about their influence on transformed roots that are rich reserves of secondary metabolites. This raises the question of how mycorrhizal colonization progresses in transformed roots grown in vitro and whether the mycorrhizal fungus presence influences the production of secondary metabolites. To fully understand mycorrhizal ontogenesis and its effect on root morphology, root biomass, total phenolics, rosmarinic acid, caffeic acid and antioxidant production under in vitro conditions, a co-culture was developed between three Agrobacterium rhizogenes-derived, elite-transformed root lines of Ocimum basilicum and Rhizophagus irregularis. We found that mycorrhizal ontogenesis in transformed roots was similar to mycorrhizal roots obtained from an in planta system. Mycorrhizal establishment was also found to be transformed root line-specific. Colonization of transformed roots increased the concentration of rosmarinic acid, caffeic acid and antioxidant production while no effect was observed on root morphological traits and biomass. Enhancement of total phenolics and rosmarinic acid in the three mycorrhizal transformed root lines was found to be transformed root line-specific and age dependent. We reveal the potential of R. irregularis as a biotic elicitor in vitro and propose its incorporation into commercial in vitro secondary metabolite production via transformed roots.
Language eng
DOI 10.1007/s00572-016-0721-4
Field of Research 060505 Mycology
060799 Plant Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085575

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