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Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid

Markworth, James F., Kaur, Gunveen, Miller, Eliza G., Larsen, Amy E., Sinclair, Andrew J., Maddipati, Krishna Rao and Cameron-Smith, David 2016, Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid, FASEB journal, vol. 30, no. 11, pp. 3714-3725, doi: 10.1096/fj.201600360R.

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Title Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid
Author(s) Markworth, James F.
Kaur, GunveenORCID iD for Kaur, Gunveen orcid.org/0000-0002-6250-0495
Miller, Eliza G.
Larsen, Amy E.
Sinclair, Andrew J.
Maddipati, Krishna Rao
Cameron-Smith, David
Journal name FASEB journal
Volume number 30
Issue number 11
Start page 3714
End page 3725
Total pages 17
Publisher Federation of American Societies for Experimental Biology
Place of publication Bethesda, M.D.
Publication date 2016-11
ISSN 0892-6638
1530-6860
Keyword(s) eicosanoids
fish oil
inflammation
lipidomics
omega-3 fatty acids
Summary In contrast to the well-characterized effects of specialized proresolving lipid mediators (SPMs) derived from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), little is known about the metabolic fate of the intermediary long-chain (LC) n-3 polyunsaturated fatty acid (PUFA) docosapentaenoic acid (DPA). In this double blind crossover study, shifts in circulating levels of n-3 and n-6 PUFA-derived bioactive lipid mediators were quantified by an unbiased liquid chromatography-tandem mass spectrometry lipidomic approach. Plasma was obtained from human subjects before and after 7 d of supplementation with pure n-3 DPA, n-3 EPA or placebo (olive oil). DPA supplementation increased the SPM resolvin D5n-3DPA (RvD5n-3DPA) and maresin (MaR)-1, the DHA vicinal diol 19,20-dihydroxy-DPA and n-6 PUFA derived 15-keto-PG E2 (15-keto-PGE2). EPA supplementation had no effect on any plasma DPA or DHA derived mediators, but markedly elevated monohydroxy-eicosapentaenoic acids (HEPEs), including the e-series resolvin (RvE) precursor 18-HEPE; effects not observed with DPA supplementation. These data show that dietary n-3 DPA and EPA have highly divergent effects on human lipid mediator profile, with no overlap in PUFA metabolites formed. The recently uncovered biologic activity of n-3 DPA docosanoids and their marked modulation by dietary DPA intake reveals a unique and specific role of n-3 DPA in human physiology.-Markworth, J. F., Kaur, G., Miller, E. G., Larsen, A. E., Sinclair, A. J., Maddipati, K. R., Cameron-Smith, D. Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid.
Language eng
DOI 10.1096/fj.201600360R
Field of Research 0601 Biochemistry And Cell Biology
0606 Physiology
1116 Medical Physiology
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Federation of American Societies for Experimental Biology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085621

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