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Electrokinetic properties of lubricin antiadhesive coatings in microfluidic systems

Greene, George W., Duffy, Emer, Shallan, Aliaa, Wuethrich, Alain and Paull, Brett 2016, Electrokinetic properties of lubricin antiadhesive coatings in microfluidic systems, Langmuir, vol. 32, no. 7, pp. 1899-1908, doi: 10.1021/acs.langmuir.5b03535.

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Title Electrokinetic properties of lubricin antiadhesive coatings in microfluidic systems
Author(s) Greene, George W.ORCID iD for Greene, George W. orcid.org/0000-0003-2250-8334
Duffy, Emer
Shallan, Aliaa
Wuethrich, Alain
Paull, Brett
Journal name Langmuir
Volume number 32
Issue number 7
Start page 1899
End page 1908
Total pages 10
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2016-02
ISSN 1520-5827
Keyword(s) Adhesiveness
Dimethylpolysiloxanes
Glycoproteins
Kinetics
Lab-On-A-Chip Devices
Polytetrafluoroethylene
Silicon Dioxide
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Materials Science, Multidisciplinary
Chemistry
Materials Science
Summary Lubricin is a glycoprotein found in articular joints which has long been recognized as being an important biological boundary lubricant molecule and, more recently, an impressive antiadhesive that readily self-assembles into a well ordered, polymer brush layer on virtually any substrate. The lubricin molecule possesses an overabundance of anionic charge, a property that is atypical among antiadhesive molecules, that enables its use as a coating for applications involving electrokinetic processes such as electrophoresis and electroosmosis. Coating the surfaces of silica and polymeric microfluidic devices with self-assembled lubricin coatings affords a unique combination of excellent fouling resistance and high charge density that enables notoriously "sticky" biomolecules such as proteins to be used and controlled electrokinetically in the device without complications arising from nonspecific adsorption. Using capillary electrophoresis, we characterized the stability, uniformity, and electrokinetic properties of lubricin coatings applied to silica and PTFE capillaries over a range of run buffer pHs and when exposed to concentrated solutions of protein. In addition, we demonstrate the effectiveness of lubricin as a coating to minimize nonspecific protein adsorption in an electrokinetically controlled polydimethylsiloxane/silica microfluidic device.
Language eng
DOI 10.1021/acs.langmuir.5b03535
Field of Research 099999 Engineering not elsewhere classified
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID DE130101458
Copyright notice ©2016, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085757

Document type: Journal Article
Collection: Institute for Frontier Materials
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