Characterisation and vascular expression of nitric oxide synthase 3 in amphibians

Cameron, Melissa, Trajanovska, Sofie, Forgan, Leonard and Donald, John 2016, Characterisation and vascular expression of nitric oxide synthase 3 in amphibians, Cell and tissue research, vol. 366, no. 3, pp. 679-692, doi: 10.1007/s00441-016-2479-6.

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Title Characterisation and vascular expression of nitric oxide synthase 3 in amphibians
Author(s) Cameron, Melissa
Trajanovska, Sofie
Forgan, Leonard
Donald, JohnORCID iD for Donald, John
Journal name Cell and tissue research
Volume number 366
Issue number 3
Start page 679
End page 692
Total pages 14
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-12
ISSN 1432-0878
Keyword(s) Amphibian
Nitric oxide
Nitric oxide synthase
Summary In mammals, nitric oxide (NO) produced by nitric oxide synthase 3 (NOS3) localised in vascular endothelial cells is an important vasodilator but the presence of NOS3 in the endothelium of amphibians has been concluded to be absent, based on physiological studies. In this study, a nos3 cDNA was sequenced from the toad, Rhinella marina. The open reading frame of R. marina nos3 encoded an 1170 amino acid protein that showed 81 % sequence identity to the recently cloned Xenopus tropicalis nos3. Rhinella marina nos3 mRNA was expressed in a range of tissues and in the dorsal aorta and pulmonary, mesenteric, iliac and gastrocnemius arteries. Furthermore, nos3 mRNA was expressed in the aorta of Xenopus laevis and X. tropicalis. Quantitative real-time PCR showed that removal of the endothelium of the lateral aorta of R. marina significantly reduced the expression of nos3 mRNA compared to control aorta with the endothelium intact. However, in situ hybridisation was not able to detect any nos3 mRNA in the dorsal aorta of R. marina. Immunohistochemistry using a homologous R. marina NOS3 antibody showed immunoreactivity (IR) within the basal region of many endothelial cells of the dorsal aorta and iliac artery. NOS3-IR was also observed in the proximal tubules and collecting ducts of the kidney but not within the capillaries of the glomeruli. This is the first study to demonstrate that vascular endothelial cells of an amphibian express NOS3.
Language eng
DOI 10.1007/s00441-016-2479-6
Field of Research 060603 Animal Physiology - Systems
1116 Medical Physiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
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