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Overexpression of striated muscle activator of Rho signaling (STARS) increases C2C12 skeletal muscle cell differentiation

Wallace, Marita A., Della Gatta, Paul A., Mir, Bilal Ahmad, Kowalski, Greg M., Kloehn, Joachim, McConville, Malcom J., Russell, Aaron P. and Lamon, Severine 2016, Overexpression of striated muscle activator of Rho signaling (STARS) increases C2C12 skeletal muscle cell differentiation, Frontiers in physiology, vol. 7, Article number : 7, pp. 1-13, doi: 10.3389/fphys.2016.00007.

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Title Overexpression of striated muscle activator of Rho signaling (STARS) increases C2C12 skeletal muscle cell differentiation
Author(s) Wallace, Marita A.
Della Gatta, Paul A.ORCID iD for Della Gatta, Paul A. orcid.org/0000-0003-2231-8370
Mir, Bilal Ahmad
Kowalski, Greg M.
Kloehn, Joachim
McConville, Malcom J.
Russell, Aaron P.ORCID iD for Russell, Aaron P. orcid.org/0000-0002-7323-9501
Lamon, SeverineORCID iD for Lamon, Severine orcid.org/0000-0002-3271-6551
Journal name Frontiers in physiology
Volume number 7
Season Article number : 7
Start page 1
End page 13
Total pages 13
Publisher Frontiers Research Foundation
Place of publication Lausanne, Switzerland
Publication date 2016
ISSN 1664-042X
Keyword(s) differentiation
myogenesis
proliferation
regeneration
skeletal muscle
Summary BACKGROUND: Skeletal muscle growth and regeneration depend on the activation of satellite cells, which leads to myocyte proliferation, differentiation and fusion with existing muscle fibers. Skeletal muscle cell proliferation and differentiation are tightly coordinated by a continuum of molecular signaling pathways. The striated muscle activator of Rho signaling (STARS) is an actin binding protein that regulates the transcription of genes involved in muscle cell growth, structure and function via the stimulation of actin polymerization and activation of serum-response factor (SRF) signaling. STARS mediates cell proliferation in smooth and cardiac muscle models; however, whether STARS overexpression enhances cell proliferation and differentiation has not been investigated in skeletal muscle cells.

RESULTS: We demonstrate for the first time that STARS overexpression enhances differentiation but not proliferation in C2C12 mouse skeletal muscle cells. Increased differentiation was associated with an increase in the gene levels of the myogenic differentiation markers Ckm, Ckmt2 and Myh4, the differentiation factor Igf2 and the myogenic regulatory factors (MRFs) Myf5 and Myf6. Exposing C2C12 cells to CCG-1423, a pharmacological inhibitor of SRF preventing the nuclear translocation of its co-factor MRTF-A, had no effect on myotube differentiation rate, suggesting that STARS regulates differentiation via a MRTF-A independent mechanism.

CONCLUSION: These findings position STARS as an important regulator of skeletal muscle growth and regeneration.
Language eng
DOI 10.3389/fphys.2016.00007
Field of Research 110699 Human Movement and Sports Science not elsewhere classified
Socio Economic Objective 920116 Skeletal System and Disorders (incl. Arthritis)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085824

Document type: Journal Article
Collections: School of Exercise and Nutrition Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.