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Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and poly(Dimethylsiloxane) (PDMS) polymers

Halimoon, Hazwan, Hussain, Abdul Rashid, Kouzani, Abbas and Alam, Muhd Nazrul Hisham Zainal 2016, Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and poly(Dimethylsiloxane) (PDMS) polymers, Sains Malaysiana, vol. 45, no. 6, pp. 969-976.

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Title Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and poly(Dimethylsiloxane) (PDMS) polymers
Author(s) Halimoon, Hazwan
Hussain, Abdul Rashid
Kouzani, AbbasORCID iD for Kouzani, Abbas orcid.org/0000-0002-6292-1214
Alam, Muhd Nazrul Hisham Zainal
Journal name Sains Malaysiana
Volume number 45
Issue number 6
Start page 969
End page 976
Total pages 8
Publisher National University of Malaysia Press
Place of publication [Kuala Lumpur, Malaysia]
Publication date 2016-06-01
ISSN 0126-6039
Keyword(s) fermentation
polymer
experiment
cost
bioreactor
oxygen
temperature
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
miniature bioreactors
online UV detection
scale down
yeast fermentation
Summary In this paper, a minibioreactor platform made of low cost polymers is presented. The minibioreactor prototype was designed as an alternative solution for carrying out microbial fermentation experiments in laboratory. The minibioreactor prototype has a working volume of 1.5 mL and was fabricated from poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) polymers. Cell density was measured online whilst agitation rates and the temperature of the reactor content can be tightly controlled to desired set-point values. As proof-of-concept, various S. cerevisae fermentation experiments were conducted. In every experiment, the minibioreactor operated stably for the entire length of operation which was nearly 40 h with very minimal volume loss i.e. about 2.8 μ·h-1 at 37°C. The minibioreactor has the maximum oxygen transfer rate (OTR) of 16.6 mmol·L-1·h-1 under the agitation rate of 300 rpm. Under these conditions, cell specific growth rate as high as 0.291 h-1 was obtained. The experimental data in the minibioreactor operation was also reproducible using shake flask where similar growth profiles were attained under a similar growth conditions.
Language eng
Field of Research 091306 Microelectromechanical Systems (MEMS)
Socio Economic Objective 970109 Expanding Knowledge in Engineering
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, National University of Malaysia Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085827

Document type: Journal Article
Collection: School of Engineering
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