Functional analysis of a Type-I ribosome inactivating protein balsamin from Momordica balsamina with anti-microbial and DNase activity

Ajji, Parminder Kaur, Walder, Ken and Puri, Munish 2016, Functional analysis of a Type-I ribosome inactivating protein balsamin from Momordica balsamina with anti-microbial and DNase activity, Plant foods for human nutrition, vol. 71, no. 3, pp. 265-271, doi: 10.1007/s11130-016-0555-4.

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Title Functional analysis of a Type-I ribosome inactivating protein balsamin from Momordica balsamina with anti-microbial and DNase activity
Formatted title Functional analysis of a Type-I ribosome inactivating protein balsamin from Momordica balsamina with anti-microbial and DNase activity
Author(s) Ajji, Parminder Kaur
Walder, KenORCID iD for Walder, Ken orcid.org/0000-0002-6758-4763
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Plant foods for human nutrition
Volume number 71
Issue number 3
Start page 265
End page 271
Total pages 7
Publisher Springer
Place of publication New York, N.Y.
Publication date 2016-09
ISSN 0921-9668
1573-9104
Keyword(s) antioxidant
antibacterial
bioactive
purification
therapeutic
nutraceutical
Summary Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg(2+) (10-50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
Language eng
DOI 10.1007/s11130-016-0555-4
Field of Research 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics)
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085835

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