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Isolation and polyphasic characterization of a novel hyper catalase producing thermophilic bacterium for the degradation of hydrogen peroxide.

Sooch, Balwinder Singh, Kauldhar, Baljinder Singh and Puri, Munish 2016, Isolation and polyphasic characterization of a novel hyper catalase producing thermophilic bacterium for the degradation of hydrogen peroxide., Bioprocess and biosystems engineering, vol. 39, no. 11, pp. 1759-1773, doi: 10.1007/s00449-016-1651-4.

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Title Isolation and polyphasic characterization of a novel hyper catalase producing thermophilic bacterium for the degradation of hydrogen peroxide.
Author(s) Sooch, Balwinder Singh
Kauldhar, Baljinder Singh
Puri, Munish
Journal name Bioprocess and biosystems engineering
Volume number 39
Issue number 11
Start page 1759
End page 1773
Total pages 15
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-07-23
ISSN 1615-7605
Keyword(s) Catalase
Degradation
Geobacillus
H2O2
Thermophile
Summary A newly isolated microbial strain of thermophilic genus Geobacillus has been described with emphasis on polyphasic characterization and its application for degradation of hydrogen peroxide. The validation of this thermophilic strain of genus Geobacillus designated as BSS-7 has been demonstrated by polyphasic taxonomy approaches through its morphological, biochemical, fatty acid methyl ester profile and 16S rDNA sequencing. This thermophilic species of Geobacillus exhibited growth at broad pH and temperature ranges coupled with production of extraordinarily high quantities of intracellular catalase, the latter of which as yet not been reported in any member of this genus. The isolated thermophilic bacterial culture BSS-7 exhibited resistance against a variety of organic solvents. The immobilized whole cells of the bacterium successfully demonstrated the degradation of hydrogen peroxide (H2O2) in a packed bed reactor. This strain has potential application in various analytical and diagnostic methods in the form of biosensors and biomarkers in addition to applications in the textile, paper, food and pharmaceutical industries.
Language eng
DOI 10.1007/s00449-016-1651-4
Field of Research 090409 Wastewater Treatment Processes
100302 Bioprocessing, Bioproduction and Bioproducts
090399 Biomedical Engineering not elsewhere classified
100199 Agricultural Biotechnology not elsewhere classified
090499 Chemical Engineering not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085836

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