Genome editing in zebrafish: a practical overview

Sertori, Robert, Trengove, Monique, Basheer, Faiza, Ward, Alister C. and Liongue, Clifford 2016, Genome editing in zebrafish: a practical overview, Briefings in functional genomics, vol. 15, no. 4, pp. 322-330, doi: 10.1093/bfgp/elv051.

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Title Genome editing in zebrafish: a practical overview
Author(s) Sertori, Robert
Trengove, Monique
Basheer, Faiza
Ward, Alister C.ORCID iD for Ward, Alister C. orcid.org/0000-0001-7945-7975
Liongue, CliffordORCID iD for Liongue, Clifford orcid.org/0000-0002-1646-4905
Journal name Briefings in functional genomics
Volume number 15
Issue number 4
Start page 322
End page 330
Total pages 9
Publisher Oxford University Press
Place of publication Oxford, Eng.
Publication date 2016
ISSN 2041-2649
2041-2657
Keyword(s) zebrafish
genome editing
zinc finger nucleases
TALENSs
CRISPR
HDR
Summary Zebrafish is a powerfulmodel for the study of vertebrate development, being amenable to a wide range of genetic and other manipulations to probe themolecular basis of development and its perturbation in disease. Over recent years, genome editing approaches have become increasingly used as an efficient and sophisticated approach to precisely engineer the zebrafish genome, which has further enhanced the utility of this organism. This review provides a practical overview of genome editing and its application in zebrafish research, including alternate strategies for introducing and screening for specific genetic changes.
Language eng
DOI 10.1093/bfgp/elv051
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085967

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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