Evaluation of glucose dehydrogenase and pyrroloquinoline quinine (pqq) mutagenesis that renders functional inadequacies in host plants

Naveed, Muhammad, Sohail, Younas, Khalid, Nauman, Ahmed, Iftikhar and Mumtaz, Abdul Samad 2015, Evaluation of glucose dehydrogenase and pyrroloquinoline quinine (pqq) mutagenesis that renders functional inadequacies in host plants, Journal of microbiology and biotechnology, vol. 25, no. 8, pp. 1349-1360, doi: 10.4014/jmb.1501.01075.

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Title Evaluation of glucose dehydrogenase and pyrroloquinoline quinine (pqq) mutagenesis that renders functional inadequacies in host plants
Author(s) Naveed, Muhammad
Sohail, Younas
Khalid, NaumanORCID iD for Khalid, Nauman orcid.org/0000-0002-8045-199X
Ahmed, Iftikhar
Mumtaz, Abdul Samad
Journal name Journal of microbiology and biotechnology
Volume number 25
Issue number 8
Start page 1349
End page 1360
Total pages 12
Publisher Korean Society for Microbiology and Biotechnology
Place of publication Seoul, Korea
Publication date 2015-08
ISSN 1738-8872
Keyword(s) glucose dehydrogenase
pyrroloquinoline quinine (pqq)
rhizosphere
Pseudomonas
mutagenesis
phosphate solubilization
plant growth promotion
Summary The rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In the recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonas strains were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU67 and P. putida QAU90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the pyrroloquinoline quinine (PQQ)- dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized their possible role as antioxidants as well as in growth promotion, as probed in vitro in lettuce and in vivo in rice, bean, and tomato plants. The results showed significant differences (p ≤ 0.05) in parameters of plant height, fresh weight, and dry weight, etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence of the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.
Language eng
DOI 10.4014/jmb.1501.01075
Field of Research 060504 Microbial Ecology
050303 Soil Biology
Socio Economic Objective 860302 Organic Fertilisers
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Korean Society for Microbiology and Biotechnology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30086038

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