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Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste

Ahmad, Nazir, Ahmed, Iftikhar, Shahzad, Armghan, Khalid, Nauman, Mehboob, Farrakh, Ahad, Karam and Muhammad Ali, Ghulam 2014, Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste, Journal of the Korean society for applied biological chemistry, vol. 57, no. 3, pp. 341-346, doi: 10.1007/s13765-013-4045-1.

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Title Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste
Author(s) Ahmad, Nazir
Ahmed, Iftikhar
Shahzad, Armghan
Khalid, NaumanORCID iD for Khalid, Nauman orcid.org/0000-0002-8045-199X
Mehboob, Farrakh
Ahad, Karam
Muhammad Ali, Ghulam
Journal name Journal of the Korean society for applied biological chemistry
Volume number 57
Issue number 3
Start page 341
End page 346
Total pages 6
Publisher Springer
Place of publication Seoul, Republic of South Korea
Publication date 2014-06
ISSN 1738-2203
2234-344X
Keyword(s) biodegradation
bioremediation
industrial waste
phenol
Pseudomonas
16S rRNA gene
Summary Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at 750 mg L-1 phenol. The strain showed complete degradation of 750 mg L-1 phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of 28 mg L-1h-1. The doubling time was recorded approximately as 12.49 h-1. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.
Language eng
DOI 10.1007/s13765-013-4045-1
Field of Research 100305 Industrial Microbiology (incl Biofeedstocks)
100203 Bioremediation
Socio Economic Objective 961407 Urban and Industrial Soils
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, The Korean Society for Applied Biological Chemistry & 2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30086056

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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