Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing

Naveed, Muhammad, Mubeen, Samavia, Khan, Sami Ullah, Ahmed, Iftikhar, Khalid, Nauman, Suleria, Hafiz Ansar Rasul, Bano, Asghari and Mumtaz, Abdul Samad 2014, Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing, Brazilian journal of microbiology, vol. 45, no. 3, pp. 985-993.

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Title Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing
Author(s) Naveed, Muhammad
Mubeen, Samavia
Khan, Sami Ullah
Ahmed, Iftikhar
Khalid, NaumanORCID iD for Khalid, Nauman
Suleria, Hafiz Ansar Rasul
Bano, Asghari
Mumtaz, Abdul Samad
Journal name Brazilian journal of microbiology
Volume number 45
Issue number 3
Start page 985
End page 993
Total pages 9
Publisher Brazilian Society for Microbiology (Sociedade Brasileira de Microbiologia)
Place of publication Sao Paolo, Brazil
Publication date 2014-07
ISSN 1678-4405
Keyword(s) rhizosphere
root nodules
16S rRNA gene sequence
glucose dehydrogenase
pyrroloquinolone quinone
Summary In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh) gene that involved in the phosphate solublization using cofactor pyrroloquinolone quinone (PQQ). Seven rhizoshperic and 3 root nodulating stains are positive for gdh gene. Furthermore, this study confirms a novel association between microbes and their hosts like field grown crops, leguminous and non-leguminous plants. It was concluded that a diverse group of bacterial population exist in the rhizosphere and root nodules that might be useful in evaluating the mechanisms behind plant microbial interactions and strains QAU-63 and QAU-68 have sequence similarity of 97 and 95% which might be declared as novel after further taxonomic characterization.
Language eng
Field of Research 060504 Microbial Ecology
0605 Microbiology
1108 Medical Microbiology
Socio Economic Objective 960805 Flora
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Sociedade Brasileira de Microbiologia
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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