Preparation and characterization of water-in-oil-in-water emulsions containing a high concentration of L-ascorbic acid
Khalid, Nauman, Kobayashi, Isao, Neves, Marcos A., Uemura, Kunihiko and Nakajima, Mitsutoshi 2013, Preparation and characterization of water-in-oil-in-water emulsions containing a high concentration of L-ascorbic acid, Bioscience, biotechnology, and biochemistry, vol. 77, no. 6, pp. 1171-1178, doi: 10.1271/bbb.120870.
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Title
Preparation and characterization of water-in-oil-in-water emulsions containing a high concentration of L-ascorbic acid
This study sought to encapsulate a high concentration of L-ascorbic acid, up to 30% (w/v), in the inner aqueous phase of water-in-oil-water (W/O/W) emulsions with soybean oil as the oil phase. Two-step homogenization was conducted to prepare W/O/W emulsions stabilized by a hydrophobic emulsifier and 30% (v/v) of W/O droplets stabilized by a hydrophilic emulsifier. First-step homogenization prepared W/O emulsions with an average aqueous droplet diameter of 2.0 to 3.0 μm. Second-step homogenization prepared W/O/W emulsions with an average W/O droplet diameter of 14 to 18 μm and coefficients of variation (CVs) of 18% to 25%. The results indicated that stable W/O/W emulsions containing a high concentration of L-ascorbic acid were obtained by adding gelatin and magnesium sulfate in the inner aqueous phase and glucose in both aqueous phases. L-Ascorbic acid retention in the W/O/W emulsions was 40% on day 30 and followed first-order kinetics.
Language
eng
DOI
10.1271/bbb.120870
Field of Research
090802 Food Engineering 0601 Biochemistry And Cell Biology 0703 Crop And Pasture Production
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