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Apicoplast-localized lysophosphatidic acid precursor assembly is required for bulk phospholipid synthesis in toxoplasma gondii and relies on an algal/plant-like glycerol 3-phosphate acyltransferase

Amiar, Souad, MacRae, James I., Callahan, Damien L., Dubois, David, van Dooren, Giel G., Shears, Melanie J., Cesbron-Delauw, Marie-France, Maréchal, Eric, McConville, Malcolm J., McFadden, Geoffrey, Yamaryo-Botté, Yoshiki and Botté, Cyrille Y. 2016, Apicoplast-localized lysophosphatidic acid precursor assembly is required for bulk phospholipid synthesis in toxoplasma gondii and relies on an algal/plant-like glycerol 3-phosphate acyltransferase, PLoS pathogens, vol. 12, no. 8, Article number: e1005765, pp. 1-30, doi: 10.1371/journal.ppat.1005765.

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Title Apicoplast-localized lysophosphatidic acid precursor assembly is required for bulk phospholipid synthesis in toxoplasma gondii and relies on an algal/plant-like glycerol 3-phosphate acyltransferase
Formatted title Apicoplast-localized lysophosphatidic acid precursor assembly is required for bulk phospholipid synthesis in toxoplasma gondii and relies on an algal/plant-like glycerol 3-phosphate acyltransferase
Author(s) Amiar, Souad
MacRae, James I.
Callahan, Damien L.ORCID iD for Callahan, Damien L. orcid.org/0000-0002-6384-8717
Dubois, David
van Dooren, Giel G.
Shears, Melanie J.
Cesbron-Delauw, Marie-France
Maréchal, Eric
McConville, Malcolm J.
McFadden, Geoffrey
Yamaryo-Botté, Yoshiki
Botté, Cyrille Y.
Journal name PLoS pathogens
Volume number 12
Issue number 8
Season Article number: e1005765
Start page 1
End page 30
Total pages 30
Publisher PLoS
Place of publication San Francisco, Calif.
Publication date 2016-08-04
ISSN 1553-7366
1553-7374
Summary Most apicomplexan parasites possess a non-photosynthetic plastid (the apicoplast), which harbors enzymes for a number of metabolic pathways, including a prokaryotic type II fatty acid synthesis (FASII) pathway. In Toxoplasma gondii, the causative agent of toxoplasmosis, the FASII pathway is essential for parasite growth and infectivity. However, little is known about the fate of fatty acids synthesized by FASII. In this study, we have investigated the function of a plant-like glycerol 3-phosphate acyltransferase (TgATS1) that localizes to the T. gondii apicoplast. Knock-down of TgATS1 resulted in significantly reduced incorporation of FASII-synthesized fatty acids into phosphatidic acid and downstream phospholipids and a severe defect in intracellular parasite replication and survival. Lipidomic analysis demonstrated that lipid precursors are made in, and exported from, the apicoplast for de novo biosynthesis of bulk phospholipids. This study reveals that the apicoplast-located FASII and ATS1, which are primarily used to generate plastid galactolipids in plants and algae, instead generate bulk phospholipids for membrane biogenesis in T. gondii.
Language eng
DOI 10.1371/journal.ppat.1005765
Field of Research 030104 Immunological and Bioassay Methods
060501 Bacteriology
110704 Cellular Immunology
0605 Microbiology
1107 Immunology
1108 Medical Microbiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30086159

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Created: Fri, 16 Sep 2016, 13:09:23 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.