You are not logged in.

Development and application of SINE multilocus and quantitative genetic markers to study oilseed rape (Brassica napus L.) crops

Allnutt, T. R., Roper, K. and Henry, C. 2008, Development and application of SINE multilocus and quantitative genetic markers to study oilseed rape (Brassica napus L.) crops, Journal of agricultural and food chemistry, vol. 56, no. 2, pp. 426-432, doi: 10.1021/jf072047a.

Attached Files
Name Description MIMEType Size Downloads

Title Development and application of SINE multilocus and quantitative genetic markers to study oilseed rape (Brassica napus L.) crops
Author(s) Allnutt, T. R.
Roper, K.
Henry, C.
Journal name Journal of agricultural and food chemistry
Volume number 56
Issue number 2
Start page 426
End page 432
Total pages 7
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2008
ISSN 0021-8561
Keyword(s) base sequence
Brassica napus
Cloning, Molecular
DNA, plant
genetic markers
molecular sequence data
sequence analysis, DNA
Short Interspersed Nucleotide Elements
SINEs
molecular markers
crop purity
volunteers
taqman
real-time PCR
quantitative genetic markers
QSINEs
Summary A genetic marker system based on the S1 Short Interspersed Elements (SINEs) in the important commercial crop, oilseed rape ( Brassica napus L.) has been developed. SINEs provided a successful multilocus, dominant marker system that was capable of clearly delineating winter- and spring-type crop varieties. Sixteen of 20 varieties tested showed unique profiles from the 17 polymorphic SINE markers generated. The 3' or 5' flank region of nine SINE markers were cloned, and DNA was sequenced. In addition, one putative pre-transposition SINE allele was cloned and sequenced. Two SINE flanking sequences were used to design real-time PCR assays. These quantitative SINE assays were applied to study the genetic structure of eight fields of oilseed rape crops. Studied fields were more genetically diverse than expected for the chosen loci (mean H T = 0.23). The spatial distribution of SINE marker frequencies was highly structured in some fields, suggesting locations of volunteer impurities within the crop. In one case, the assay identified a mislabeling of the crop variety. SINE markers were a useful tool for crop genetics, phylogenetics, variety identification, and purity analysis. The use and further application of quantitative, real-time PCR markers are discussed.
Language eng
DOI 10.1021/jf072047a
Field of Research 060499 Genetics not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30086572

Document type: Journal Article
Collection: School of Medicine
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in TR Web of Science
Scopus Citation Count Cited 1 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 20 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Thu, 16 Feb 2017, 09:56:08 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.