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Isothermal amplification of genetically modified DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping

Lee, David, La Mura, Maurizio, Allnutt, Theo, Powell, Wayne and Greenland, Andy 2009, Isothermal amplification of genetically modified DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping, Journal of agricultural and food chemistry, vol. 57, no. 20, pp. 9400-9402, doi: 10.1021/jf902455j.

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Title Isothermal amplification of genetically modified DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping
Author(s) Lee, David
La Mura, Maurizio
Allnutt, Theo
Powell, Wayne
Greenland, Andy
Journal name Journal of agricultural and food chemistry
Volume number 57
Issue number 20
Start page 9400
End page 9402
Total pages 3
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2009-10-28
ISSN 1520-5118
Keyword(s) DNA, Plant
genotype
high-throughput screening assays
nucleic acid amplification techniques
zea mays
loop-mediated isothermal amplification (LAMP)
GM detection
RF3 oilseed rape
leaf tissue
MON810 maize seed
Summary DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures.
Language eng
DOI 10.1021/jf902455j
Field of Research 060499 Genetics not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30086576

Document type: Journal Article
Collection: School of Medicine
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