Validation of a real-time PCR on-site quantification method for MON810 maize

Langrell, Stephen R. H., Allnutt, Theodore R., Laval, Valérie, Bertheau, Yves, Pla, Maria, Papazova, Nina and Lee, David 2011, Validation of a real-time PCR on-site quantification method for MON810 maize, Food analytical methods, vol. 4, no. 3, pp. 313-318, doi: 10.1007/s12161-010-9167-z.

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Title Validation of a real-time PCR on-site quantification method for MON810 maize
Author(s) Langrell, Stephen R. H.
Allnutt, Theodore R.ORCID iD for Allnutt, Theodore R.
Laval, Valérie
Bertheau, Yves
Pla, Maria
Papazova, Nina
Lee, David
Journal name Food analytical methods
Volume number 4
Issue number 3
Start page 313
End page 318
Total pages 6
Publisher Springer
Place of publication Berlin, Germany
Publication date 2011-09
ISSN 1936-9751
Summary A rapid on-, or near-site, quantitative method for use as a pre-harvest predictive decision, or co-existence monitoring, tool for adventitious genetically modified (GM) presence has been developed. Based on a laboratory-based protocol for real-time (RT) quantification of the MON810 GM event in maize kernels, the duplex RT polymerase chain reaction method was constructed around the portable Cepheid SmartCyclerII platform, requiring only modest support infrastructure for field application. Validation through an international ring trial showed good compliance with minimum assay performance requirements as defined by the European Network of GMO Laboratories (RSD r=18.5%; RSD R=32.8; Bias=26.7%). © 2010 Springer Science+Business Media, LLC.
Language eng
DOI 10.1007/s12161-010-9167-z
Field of Research 090899 Food Sciences not elsewhere classified
0908 Food Sciences
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2010, Springer Science+Business Media, LLC
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Document type: Journal Article
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School of Medicine
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