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Development of a microsatellite-based method for the differentiation of European wild boar (Sus scrofa scrofa) from domestic pig breeds (Sus scrofa domestica) in food

Conyers, Christine M., Allnutt, Theodore R., Hird, Heather J., Kaye, Joy and Chisholm, James 2012, Development of a microsatellite-based method for the differentiation of European wild boar (Sus scrofa scrofa) from domestic pig breeds (Sus scrofa domestica) in food, Journal of agricultural and food chemistry, vol. 60, no. 13, pp. 3341-3347, doi: 10.1021/jf205109b.

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Title Development of a microsatellite-based method for the differentiation of European wild boar (Sus scrofa scrofa) from domestic pig breeds (Sus scrofa domestica) in food
Author(s) Conyers, Christine M.
Allnutt, Theodore R.
Hird, Heather J.
Kaye, Joy
Chisholm, James
Journal name Journal of agricultural and food chemistry
Volume number 60
Issue number 13
Start page 3341
End page 3347
Total pages 7
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2012-04
ISSN 0021-8561
1520-5118
Keyword(s) Animals
Breeding
Europe
Food Analysis
Food Contamination
Genotype
Meat Products
Microsatellite Repeats
Quality Control
Sus scrofa
Summary Twenty microsatellites (simple sequence repeats, SSR) were used to discriminate wild boar from domestic pig and to identify mixtures of the two. Reference groups of wild boar and pig samples were collected from the UK and Europe for genetic assignment tests. Bayesian Analysis of Populations software (BAPs) gave 100% correct assignment for blind wild boar and pig samples and correctly identified mixed samples. DNA was extracted from 12 commercial food samples (11 labeled as containing wild boar) including patés, salamis, and sausage, and good SSR profiles were obtained. Eleven samples were correctly assigned as pig, and two as mixed meats. One sample sold as wild boar meat was clearly assigned as pig. A further 10 blind samples of meat cuts were analyzed, eight wild boar and two pig, and all were correctly assigned.
Language eng
DOI 10.1021/jf205109b
Field of Research 060499 Genetics not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30086581

Document type: Journal Article
Collection: School of Medicine
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