Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults Lamon, Severine, Zacharewicz, Evelyn, Arentson-Lantz, Emily, Della Gatta, Paul A., Ghobrial, Lobna, Gerlinger Romero, Frederico, Garnham, Andrew, Paddon-Jones, Douglas and Russell, Aaron P. 2016, Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults, Frontiers in physiology, vol. 7, Article number : 292, pp. 1-8, doi: 10.3389/fphys.2016.00292. Attached Files Name Description MIMEType Size Downloads lamon-erythropoietindoesnot-2016.pdf Published version application/pdf 1.84MB 8 Title Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults Author(s) Lamon, Severine orcid.org/0000-0002-3271-6551 Zacharewicz, Evelyn Arentson-Lantz, Emily Della Gatta, Paul A. orcid.org/0000-0003-2231-8370 Ghobrial, Lobna Gerlinger Romero, Frederico orcid.org/0000-0002-2150-0384 Garnham, Andrew Paddon-Jones, Douglas Russell, Aaron P. orcid.org/0000-0002-7323-9501 Journal name Frontiers in physiology Volume number 7 Season Article number : 292 Start page 1 End page 8 Total pages 8 Publisher Frontiers Research Foundation Place of publication Lausanne, Switzerland Publication date 2016-07 Keyword(s) aging anabolic signaling erythropoietin muscle protein synthesis resistance exercise Summary PURPOSE: Erythropoietin (EPO) is a renal cytokine that is primarily involved in hematopoiesis while also playing a role in non-hematopoietic tissues expressing the EPO-receptor (EPOR). The EPOR is present in human skeletal muscle. In mouse skeletal muscle, EPO stimulation can activate the AKT serine/threonine kinase 1 (AKT) signaling pathway, the main positive regulator of muscle protein synthesis. We hypothesized that a single intravenous EPO injection combined with acute resistance exercise would have a synergistic effect on skeletal muscle protein synthesis via activation of the AKT pathway. METHODS: Ten young (24.2 ± 0.9 years) and 10 older (66.6 ± 1.1 years) healthy subjects received a primed, constant infusion of [ring-13C(6)] L-phenylalanine and a single injection of 10,000 IU epoetin-beta or placebo in a double-blind randomized, cross-over design. 2 h after the injection, the subjects completed an acute bout of leg extension resistance exercise to stimulate skeletal muscle protein synthesis. RESULTS: Significant interaction effects in the phosphorylation levels of the members of the AKT signaling pathway indicated a differential activation of protein synthesis signaling in older subjects when compared to young subjects. However, EPO offered no synergistic effect on vastus lateralis mixed muscle protein synthesis rate in young or older subjects. CONCLUSIONS: Despite its ability to activate the AKT pathway in skeletal muscle, an acute EPO injection had no additive or synergistic effect on the exercise-induced activation of muscle protein synthesis or muscle protein synthesis signaling pathways. Language eng DOI 10.3389/fphys.2016.00292 Field of Research 110602 Exercise Physiology Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2016, The Authors Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30087666 Document type: Journal Article Collections: School of Exercise and Nutrition Sciences Open Access Collection Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 20 Oct 2016, 09:27:00 EST Thu, 27 Oct 2016, 08:12:23 EST Thu, 24 Nov 2016, 05:04:10 EST Mon, 28 Nov 2016, 14:22:31 EST Mon, 28 Nov 2016, 14:30:53 EST Fri, 03 Mar 2017, 15:33:52 EST Filtered Full Citation counts:   Cited 1 times in TR Web of Science Cited 1 times in Scopus Search Google Scholar Access Statistics: 127 Abstract Views, 9 File Downloads  -  Detailed Statistics Created: Thu, 20 Oct 2016, 09:27:00 EST

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