You are not logged in.
Openly accessible

Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria

Strauss, Juliette A., Shaw, Cristopher S., Bradley, Helen, Wilson, Oliver J., Dorval, Thierry, Pilling, James and Wagenmakers, Anton J. M. 2016, Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria, Physiological reports, vol. 4, no. 1, Article number : e12662, pp. 1-11, doi: 10.14814/phy2.12662.

Attached Files
Name Description MIMEType Size Downloads
shaw-immunofluorescence-2016.pdf Published version application/pdf 2.81MB 3

Title Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria
Author(s) Strauss, Juliette A.
Shaw, Cristopher S.ORCID iD for Shaw, Cristopher S. orcid.org/0000-0003-1499-0220
Bradley, Helen
Wilson, Oliver J.
Dorval, Thierry
Pilling, James
Wagenmakers, Anton J. M.
Journal name Physiological reports
Volume number 4
Issue number 1
Season Article number : e12662
Start page 1
End page 11
Total pages 11
Publisher Wiley-Blackwell
Place of publication Chichester, Eng.
Publication date 2016
ISSN 2051-817X
Keyword(s) Glucose transporter 4
intramuscular triglyceride
lipid droplets
mitochondria
synaptosomal‐associated protein 23
Cell Membrane
Humans
Male
Microscopy, Fluorescence
Muscle, Skeletal
Qb-SNARE Proteins
Qc-SNARE Proteins
Young Adult
Summary Synaptosomal-associated protein 23 (SNAP23) is a SNARE protein expressed abundantly in human skeletal muscle. Its established role is to mediate insulin-stimulated docking and fusion of glucose transporter 4 (GLUT4) with the plasma membrane. Recent in vitro research has proposed that SNAP23 may also play a role in the fusion of growing lipid droplets (LDs) and the channeling of LD-derived fatty acids (FAs) into neighboring mitochondria for β-oxidation. This study investigates the subcellular distribution of SNAP23 in human skeletal muscle using immunofluorescence microscopy to confirm that SNAP23 localization supports the three proposed metabolic roles. Percutaneous biopsies were obtained from the m. vastus lateralis of six lean, healthy males in the rested, overnight fasted state. Cryosections were stained with antibodies targeting SNAP23, the mitochondrial marker cytochrome c oxidase and the plasma membrane marker dystrophin, whereas intramuscular LDs were stained using the neutral lipid dye oil red O. SNAP23 displayed areas of intense punctate staining in the intracellular regions of all muscle fibers and continuous intense staining in peripheral regions of the cell. Quantitation of confocal microscopy images showed colocalization of SNAP23 with the plasma membrane marker dystrophin (Pearson's correlation coefficient r = 0.50 ± 0.01). The intense punctate intracellular staining colocalized primarily with the mitochondrial marker cytochrome C oxidase (r = 0.50 ± 0.012) and to a lesser extent with LDs (r = 0.21 ± 0.01) visualized with oil red O. We conclude that the observed subcellular distribution of SNAP23 in human skeletal muscle supports the three aforementioned metabolic roles.
Language eng
DOI 10.14814/phy2.12662
Field of Research 110602 Exercise Physiology
110699 Human Movement and Sports Science not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30087864

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 2 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 35 Abstract Views, 4 File Downloads  -  Detailed Statistics
Created: Thu, 20 Oct 2016, 12:25:57 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.