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First comprehensive multi-tissue transcriptome of Cherax quadricarinatus (Decapoda: Parastacidae) reveals unexpected diversity of endogenous cellulase

Tan, Mun Hua, Gan, Han Ming, Gan, Huan You, Lee, Yin Peng, Croft, Larry J., Schultz, Mark B., Miller, Adam D. and Austin, Christopher M. 2016, First comprehensive multi-tissue transcriptome of Cherax quadricarinatus (Decapoda: Parastacidae) reveals unexpected diversity of endogenous cellulase, Organisms diversity and evolution, vol. 16, no. 1, pp. 185-200, doi: 10.1007/s13127-015-0237-3.

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Title First comprehensive multi-tissue transcriptome of Cherax quadricarinatus (Decapoda: Parastacidae) reveals unexpected diversity of endogenous cellulase
Author(s) Tan, Mun Hua
Gan, Han Ming
Gan, Huan You
Lee, Yin Peng
Croft, Larry J.
Schultz, Mark B.
Miller, Adam D.ORCID iD for Miller, Adam D. orcid.org/0000-0002-1632-7206
Austin, Christopher M.
Journal name Organisms diversity and evolution
Volume number 16
Issue number 1
Start page 185
End page 200
Total pages 16
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016
ISSN 1439-6092
1618-1077
Keyword(s) freshwater crayfish
genomics
pancrustacea
cellulase
Summary The Australian freshwater crayfish species, Cherax quadricarinatus Von Martens, 1868, is an important commercial and invasive species that is also being increasingly used as a model organism to address important and interesting questions in crustacean biology. Through deep sequencing of the transcriptome of C. quadricarinatus from the hepatopancreas and four other tissues, we examine the evolution of endogenously transcribed cellulase genes and provide new insights into controversial issues regarding the nutritional biology of crayfishes. A cluster assembly approach yielded one of the highest quality transcriptome assemblies for a decapod crustacean to date. A total of 206,341,872 reads with an average read length of 80 bp were generated from sequencing the transcriptomes from the heart, kidney, hepatopancreas, nerve, and testis tissues. The assembled transcriptome contains a total of 44,525 transcripts. A total of 65 transcripts coding for carbohydrate-active enzymes (CAZy) were identified based on hidden Markov model (HMM), and a majority of them display high relative transcript abundance in the hepatopancreas tissue, supporting their role in nutrient digestion. Comprehensive phylogenetic analyses of proteins belonging to two main glycosyl hydrolase families (GH9 and GH5) suggest shared ancestry of C. quadricarinatus cellulases with other characterized crustacean cellulases. Our study significantly expands the number of known crustacean-derived CAZy-coding transcripts. More importantly, the surprising level of evolutionary diversification of these proteins in C. quadricarinatus suggests that these enzymes may have been of critical importance in the adaptation of freshwater crayfishes to new plant-based food sources as part of their successful invasion of freshwater systems from marine ancestors.
Language eng
DOI 10.1007/s13127-015-0237-3
Field of Research 060408 Genomics
060405 Gene Expression (incl Microarray and other genome-wide approaches)
060802 Animal Cell and Molecular Biology
0603 Evolutionary Biology
Socio Economic Objective 960807 Fresh
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Gesellschaft für Biologische Systematik
Persistent URL http://hdl.handle.net/10536/DRO/DU:30088763

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