Probing synechocystis-arsenic interactions through extracellular nanowires.

Sure, Sandeep Karbhari, Ackland, Margaret, Gaur, Aditya, Gupta, Priyanka, Adholeya, Alok and Kochar, Mandira 2016, Probing synechocystis-arsenic interactions through extracellular nanowires., Frontiers in microbiology, vol. 7, no. 1134, pp. 1-12, doi: 10.3389/fmicb.2016.01134.

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Title Probing synechocystis-arsenic interactions through extracellular nanowires.
Author(s) Sure, Sandeep Karbhari
Ackland, MargaretORCID iD for Ackland, Margaret
Gaur, Aditya
Gupta, Priyanka
Adholeya, Alok
Kochar, Mandira
Journal name Frontiers in microbiology
Volume number 7
Issue number 1134
Start page 1
End page 12
Total pages 12
Publisher Frontiers Media S. A.
Place of publication Lausanne, Switzerland
Publication date 2016-07-19
Keyword(s) PilA
microbial nanowires
Summary Microbial nanowires (MNWs) can play an important role in the transformation and mobility of toxic metals/metalloids in environment. The potential role of MNWs in cell-arsenic (As) interactions has not been reported in microorganisms and thus we explored this interaction using Synechocystis PCC 6803 as a model system. The effect of half maximal inhibitory concentration (IC50) [~300 mM As (V) and ~4 mM As (III)] and non-inhibitory [4X lower than IC50, i.e., 75 mM As (V) and 1 mM As (III)] of As was studied on Synechocystis cells in relation to its effect on Chlorophyll (Chl) a, type IV pili (TFP)-As interaction and intracellular/extracellular presence of As. In silico analysis showed that subunit PilA1 of electrically conductive TFP, i.e., microbial nanowires of Synechocystis have putative binding sites for As. In agreement with in silico analysis, transmission electron microscopy analysis showed that As was deposited on Synechocystis nanowires at all tested concentrations. The potential of Synechocystis nanowires to immobilize As can be further enhanced and evaluated on a large scale and thus can be applied for bioremediation studies.
Language eng
DOI 10.3389/fmicb.2016.01134
Field of Research 030302 Nanochemistry and Supramolecular Chemistry
040604 Natural Hazards
100203 Bioremediation
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Frontiers Media S.A.
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