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Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids

Shrestha, Pushkar, Callahan, Damien, Singh, Surinder P, Petrie, James R and Zhou, Xue-Rong 2016, Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids, Frontiers in plant science, vol. 7, pp. 1-15, doi: 10.3389/fpls.2016.01402.

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Title Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids
Author(s) Shrestha, Pushkar
Callahan, Damien
Singh, Surinder P
Petrie, James R
Zhou, Xue-Rong
Journal name Frontiers in plant science
Volume number 7
Start page 1
End page 15
Total pages 15
Publisher Frontiers Media
Place of publication Lausanne, Switzerland
Publication date 2016-09-26
ISSN 1664-462X
Keyword(s) triacylglycerolutilization
lipidomics
3 LC-PUFA
SDA
ARA
DHA
Arabidopsis
seed germination
Summary There are now several examples of plant species engineered to synthesize and accumulate nutritionally important polyunsaturated fatty acids in their seed triacylglycerols (TAG). The utilization of TAG in germinating seeds of such transgenic plants was unknown. In this study, we examined the TAG utilization efficiency during seed germination in transgenic Arabidopsis seeds containing several examples of these fatty acids. Seed TAG species with native fatty acids had higher utilization rate than the TAG species containing transgenically produced polyunsaturated fatty acids. Conversely, quantification of the fatty acid components remaining in the total TAG after early stages of seed germination revealed that the undigested TAGs tended to contain elevated levels of the engineered polyunsaturated fatty acids (PUFA). LC-MS analysis further revealed asymmetrical mobilization rates for the individual TAG species. TAGs which contained multiple PUFA fatty acids were mobilized slower than the species containing single PUFA. The mobilized engineered fatty acids were used in de novo membrane lipid synthesis during seedling development.
Language eng
DOI 10.3389/fpls.2016.01402
Field of Research 060799 Plant Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Shrestha, Callahan, Singh, Petrie and Zhou.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089028

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