You are not logged in.

CD39 overexpression does not attenuate renal fibrosis in the unilateral ureteric obstructive model of chronic kidney disease

Roberts, Veena, Lu, B., Chia, J., Cowan, P.J. and Dwyer, K.M. 2016, CD39 overexpression does not attenuate renal fibrosis in the unilateral ureteric obstructive model of chronic kidney disease, Purinergic signalling, vol. 12, no. 4, pp. 653-660, doi: 10.1007/s11302-016-9528-1.

Attached Files
Name Description MIMEType Size Downloads

Title CD39 overexpression does not attenuate renal fibrosis in the unilateral ureteric obstructive model of chronic kidney disease
Author(s) Roberts, Veena
Lu, B.
Chia, J.
Cowan, P.J.
Dwyer, K.M.
Journal name Purinergic signalling
Volume number 12
Issue number 4
Start page 653
End page 660
Total pages 8
Publisher Springer
Place of publication Dordrecht, The Netherlands
Publication date 2016-12
ISSN 1573-9546
Keyword(s) adenosine receptor
CD39
renal fibrosis
transforming growth factor-ß
unilateral ureteric obstruction
Summary Chronic kidney disease has multiple etiologies, but its single, hallmark lesion is renal fibrosis. CD39 is a key purinergic enzyme in the hydrolysis of ATP and increased CD39 activity on regulatory T cells (Treg) is protective in adriamycin-induced renal fibrosis. We examined the effect of overexpression of human CD39 on the development of renal fibrosis in the unilateral ureteric obstructive (UUO) model, a model widely used to study the molecular and cellular factors involved in renal fibrosis. Mice overexpressing human CD39 (CD39Tg) and their wild-type (WT) littermates were subjected to UUO; renal histology and messenger RNA (mRNA) levels of adenosine receptors and markers of renal fibrosis were examined up to 14 days after UUO. There were no differences between CD39Tg mice and WT mice in the development of renal fibrosis at days 3, 7, and 14 of UUO. Relative mRNA expression of the adenosine A2A receptor and endothelin-1 were higher in CD39Tg than WT mice at day 7 post UUO, but there were no differences in markers of fibrosis. We conclude that human CD39 overexpression does not attenuate the development of renal fibrosis in the UUO model. The lack of protection by CD39 overexpression in the UUO model is multifactorial due to the different effects of adenosinergic receptors on the development of renal fibrosis.
Language eng
DOI 10.1007/s11302-016-9528-1
Field of Research 111502 Clinical Pharmacology and Therapeutics
0601 Biochemistry And Cell Biology
Socio Economic Objective 920199 Clinical Health (Organs
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer Science + Business Media Dordrecht
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089248

Document type: Journal Article
Collection: School of Medicine
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 25 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Fri, 10 Mar 2017, 08:28:08 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.