Plasma 25-hydroxyvitamin D level is related to protein signaling involved in glucose homeostasis in a tissue-specific manner Parker, Lewan, Levinger, Itamar, Mousa, Aya, Howlett, Kirsten and de Courten, Barbora 2016, Plasma 25-hydroxyvitamin D level is related to protein signaling involved in glucose homeostasis in a tissue-specific manner, Nutrients, vol. 8, no. 10, Article Number : 631, pp. 1-9, doi: 10.3390/nu8100631. Attached Files Name Description MIMEType Size Downloads howlett-plasma25hydroxyvitamin-2016.pdf Published version application/pdf 717.87KB 28 Title Plasma 25-hydroxyvitamin D level is related to protein signaling involved in glucose homeostasis in a tissue-specific manner Author(s) Parker, Lewan Levinger, Itamar Mousa, Aya Howlett, Kirsten orcid.org/0000-0002-8571-4867 de Courten, Barbora Journal name Nutrients Volume number 8 Issue number 10 Season Article Number : 631 Start page 1 End page 9 Total pages 9 Publisher MDPI Place of publication Basel, Switzerland Publication date 2016-10 ISSN 2072-6643 Keyword(s) diabetes glucose homeostasis insulin resistance insulin signaling vitamin D 25-hydroxyvitamin D Science & Technology Life Sciences & Biomedicine Nutrition & Dietetics VITAMIN-D DEFICIENCY PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY RANDOMIZED CONTROLLED-TRIAL PLACEBO-CONTROLLED TRIAL HUMAN SKELETAL-MUSCLE MIDDLE-AGED MEN INSULIN-RESISTANCE DIABETES-MELLITUS GLUT-4 PHOSPHORYLATION D SUPPLEMENTATION Summary Vitamin D has been suggested to play a role in glucose metabolism. However, previous findings are contradictory and mechanistic pathways remain unclear. We examined the relationship between plasma 25-hydroxyvitamin D (25(OH)D), insulin sensitivity, and insulin signaling in skeletal muscle and adipose tissue. Seventeen healthy adults (Body mass index: 26 ± 4; Age: 30 ± 12 years) underwent a hyperinsulinemic-euglycemic clamp, and resting skeletal muscle and adipose tissue biopsies. In this cohort, the plasma 25(OH)D concentration was not associated with insulin sensitivity (r = 0.19, p = 0.56). However, higher plasma 25(OH)D concentrations correlated with lower phosphorylation of glycogen synthase kinase-3 (GSK-3) αSer21 and βSer9 in skeletal muscle (r = −0.66, p = 0.015 and r = −0.53, p = 0.06, respectively) and higher GSK-3 αSer21 and βSer9 phosphorylation in adipose tissue (r = 0.82, p < 0.01 and r = 0.62, p = 0.042, respectively). Furthermore, higher plasma 25(OH)D concentrations were associated with greater phosphorylation of both protein kinase-B (AktSer473) (r = 0.78, p < 0.001) and insulin receptor substrate-1 (IRS-1Ser312) (r = 0.71, p = 0.01) in adipose tissue. No associations were found between plasma 25(OH)D concentration and IRS-1Tyr612 phosphorylation in skeletal muscle and adipose tissue. The divergent findings between muscle and adipose tissue with regard to the association between 25(OH)D and insulin signaling proteins may suggest a tissue-specific interaction with varying effects on glucose homeostasis. Further research is required to elucidate the physiological relevance of 25(OH)D in each tissue. Language eng DOI 10.3390/nu8100631 Field of Research 111199 Nutrition and Dietetics not elsewhere classified 1111 Nutrition And Dietetics Socio Economic Objective 920104 Diabetes HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2016, The Authors Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30089457 Document type: Journal Article Collections: Faculty of Health Institute for Physical Activity and Nutrition Open Access Collection Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 28 Nov 2016, 14:13:47 EST Tue, 29 Nov 2016, 04:01:38 EST Tue, 29 Nov 2016, 07:05:51 EST Wed, 11 Jan 2017, 09:52:56 EST Tue, 17 Jan 2017, 13:24:17 EST Sat, 28 Jan 2017, 05:06:02 EST Mon, 06 Feb 2017, 11:24:13 EST Mon, 06 Feb 2017, 11:25:52 EST Mon, 06 Feb 2017, 11:26:52 EST Thu, 09 Feb 2017, 09:03:21 EST Fri, 07 Sep 2018, 12:20:36 EST Filtered Full Citation counts:   Cited 10 times in TR Web of Science Cited 10 times in Scopus Search Google Scholar Access Statistics: 287 Abstract Views, 29 File Downloads  -  Detailed Statistics Created: Tue, 17 Jan 2017, 13:24:17 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.