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Molecular physiology reveals ammonium uptake and related gene expression in the seagrass Zostera muelleri

Pernice, Mathieu, Sinutok, Sutinee, Sablok, Gaurav, Commault, Audrey S., Schliep, Martin, Macreadie, Peter I., Rasheed, Michael A. and Ralph, Peter J. 2016, Molecular physiology reveals ammonium uptake and related gene expression in the seagrass Zostera muelleri, Marine environmental research, vol. 122, pp. 126-134, doi: 10.1016/j.marenvres.2016.10.003.

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Title Molecular physiology reveals ammonium uptake and related gene expression in the seagrass Zostera muelleri
Author(s) Pernice, Mathieu
Sinutok, Sutinee
Sablok, Gaurav
Commault, Audrey S.
Schliep, Martin
Macreadie, Peter I.ORCID iD for Macreadie, Peter I. orcid.org/0000-0001-7362-0882
Rasheed, Michael A.
Ralph, Peter J.
Journal name Marine environmental research
Volume number 122
Start page 126
End page 134
Total pages 9
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-05-10
ISSN 0141-1136
Keyword(s) seagrass
molecular physiology
isotope
nutrients
nitrogen
RT-qPCR
Summary Seagrasses are important marine foundation species, which are presently threatened by coastal development and global change worldwide. The molecular mechanisms that drive seagrass responses to anthropogenic stresses, including elevated levels of nutrients such as ammonium, remains poorly understood. Despite the evidence that seagrasses can assimilate ammonium by using glutamine synthetase (GS)/glutamate synthase (glutamine-oxoglutarate amidotransferase or GOGAT) cycle, the regulation of this fundamental metabolic pathway has never been studied at the gene expression level in seagrasses so far. Here, we combine (i) reverse transcription quantitative real-time PCR (RT-qPCR) to measure expression of key genes involved in the GS/GOGAT cycle, and (ii) stable isotope labelling and mass spectrometry to investigate 15N-ammonium assimilation in the widespread Australian species Zostera muelleri subsp. capricorni (Z. muelleri). We demonstrate that exposure to a pulse of ammonium in seawater can induce changes in GS gene expression of Z. muelleri, and further correlate these changes in gene expression with 15N-ammonium uptake rate in above- and below-ground tissue.
Language eng
DOI 10.1016/j.marenvres.2016.10.003
Field of Research 060701 Phycology (incl Marine Grasses)
03 Chemical Sciences
05 Environmental Sciences
06 Biological Sciences
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089561

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