You are not logged in.

1H magnetic resonance spectroscopy metabolites as biomarkers for cell cycle arrest and cell death in rat glioma cells

Mirbahai, Ladan, Wilson, Martin, Shaw, Christopher S., McConville, Carmel, Malcomson, Roger D.G., Griffin, Julian L., Kauppinen, Risto A. and Peet, Andrew C. 2011, 1H magnetic resonance spectroscopy metabolites as biomarkers for cell cycle arrest and cell death in rat glioma cells, International journal of biochemistry & cell biology, vol. 43, no. 7, pp. 990-1001, doi: 10.1016/j.biocel.2010.07.002.

Attached Files
Name Description MIMEType Size Downloads

Title 1H magnetic resonance spectroscopy metabolites as biomarkers for cell cycle arrest and cell death in rat glioma cells
Formatted title 1H magnetic resonance spectroscopy metabolites as biomarkers for cell cycle arrest and cell death in rat glioma cells
Author(s) Mirbahai, Ladan
Wilson, Martin
Shaw, Christopher S.ORCID iD for Shaw, Christopher S. orcid.org/0000-0003-1499-0220
McConville, Carmel
Malcomson, Roger D.G.
Griffin, Julian L.
Kauppinen, Risto A.
Peet, Andrew C.
Journal name International journal of biochemistry & cell biology
Volume number 43
Issue number 7
Start page 990
End page 1001
Total pages 12
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2011-07
ISSN 1357-2725
1878-5875
Keyword(s) biomarkers
cell growth arrest
necrosis
metabolite alterations
1H magic angle spinning NMR spectroscopy
Amino Acids
Animals
Biomarkers, Tumor
Cell Death
Cell Line, Tumor
Cisplatin
G1 Phase
Glioma
Magnetic Resonance Spectroscopy
Phosphorylcholine
Protons
Rats
Succinic Acid
Summary BACKGROUND: Improved non-invasive imaging biomarkers of treatment response contribute to optimising cancer management and metabolites detected by proton magnetic resonance spectroscopy (1H MRS) show promise in this area. Understanding 1H MRS changes occurring in cells during cell stress and cell death in vitro should aid the selection of pertinent biomarkers for clinical use.

METHODS: BT4C glioma cells in culture were exposed to either 50 μM cis-dichlorodiammineplatinum II (cisplatin) or starvation by culture in phosphate buffered saline. High resolution magic angle spinning 1H MRS was performed on cells using a Varian 600 MHz nanoprobe and metabolites were quantified by a time domain fitting method. Cell viability was assessed by trypan blue, H&E, 4',6-diamino-2-phenylindole (DAPI), DNA laddering and annexin V-FITC labelled flow cytometry; propidium iodide flow cytometry was used to assess the cell cycle phase.

RESULTS: With cisplatin exposure, cells initially accumulated in the G1 stage of the cell cycle with low numbers of apoptotic and necrotic cells and this was associated with decreases in phosphocholine, succinate, alanine, taurine, glycine and glutamate and increases in lactate and glycerophosphocholine (GPC). Starvation, leading to necrotic cell death within 6-18 h, caused decreases in succinate, alanine, glycine, and glutamate and increases in GPC. Principal component analysis revealed two patterns of metabolite changes, one common to both types of cell stress and another specific for necrosis secondary to cell starvation.

CONCLUSIONS: 1H MRS reveals alterations in multiple metabolites during cell cycle arrest and cell death which may provide early biomarker profiles of treatment efficacy in vivo.
Language eng
DOI 10.1016/j.biocel.2010.07.002
Field of Research 111202 Cancer Diagnosis
0601 Biochemistry And Cell Biology
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2010, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089566

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in TR Web of Science
Scopus Citation Count Cited 25 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 28 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 28 Nov 2016, 15:04:05 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.