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Preferential utilization of perilipin 2-associated intramuscular triglycerides during 1 h of moderate-intensity endurance-type exercise

Shepherd, S. O., Cocks, M., Tipton, K. D., Ranasinghe, A. M., Barker, T. A., Burniston, J. G., Wagenmakers, A. J. M. and Shaw, C. S. 2012, Preferential utilization of perilipin 2-associated intramuscular triglycerides during 1 h of moderate-intensity endurance-type exercise, Experimental physiology, vol. 97, no. 8, pp. 970-980, doi: 10.1113/expphysiol.2012.064592.

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Title Preferential utilization of perilipin 2-associated intramuscular triglycerides during 1 h of moderate-intensity endurance-type exercise
Author(s) Shepherd, S. O.
Cocks, M.
Tipton, K. D.
Ranasinghe, A. M.
Barker, T. A.
Burniston, J. G.
Wagenmakers, A. J. M.
Shaw, C. S.ORCID iD for Shaw, C. S. orcid.org/0000-0003-1499-0220
Journal name Experimental physiology
Volume number 97
Issue number 8
Start page 970
End page 980
Total pages 11
Publisher Wiley
Place of publication Chichester, Eng.
Publication date 2012-08
ISSN 0958-0670
1469-445X
Keyword(s) Adult
Exercise
Humans
Male
Membrane Proteins
Myosin Heavy Chains
Oxygen Consumption
Perilipin-2
Physical Endurance
Quadriceps Muscle
Triglycerides
Young Adult
Summary The lipid droplet (LD)-associated protein perilipin 2 (PLIN2) appears to colocalize with LDs in human skeletal muscle fibres, although the function of PLIN2 in the regulation of intramuscular triglyceride (IMTG) metabolism is currently unknown. Here we investigated the hypothesis that the presence of PLIN2 in skeletal muscle LDs is related to IMTG utilisation during exercise. We therefore measured exercise-induced changes in IMTG and PLIN2 distribution and changes in their colocalization. Muscle biopsies from the vastus lateralis were obtained from seven lean, untrained men (22 ± 2 years old, body mass index 24.2 ± 0.9 kg m-2 and peak oxygen uptake 3.35 ± 0.13 l min-1) before and after 1 h of moderate-intensity cycling at ~65% peak oxygen uptake. Cryosections were stained for perilipin 2, IMTG and myosin heavy chain type I and viewed using wide-field and confocal fluorescence microscopy. Exercise induced a 50 ± 7% decrease in IMTG content in type I fibres only (P < 0.05), but no change in PLIN2 content. Colocalization analysis showed that the fraction of PLIN2 associated with IMTG was 0.67 ± 0.03 before exercise, which was reduced to 0.51 ± 0.01 postexercise (P < 0.05). Further analysis revealed that the number of PLIN2-associated LDs was reduced by 31 ± 10% after exercise (P < 0.05), whereas the number of PLIN2-null LDs was unchanged. No such changes were seen in type II fibres. In conclusion, this study shows that PLIN2 content in skeletal muscle is unchanged in response to a single bout of endurance exercise. Furthermore, the PLIN2 and IMTG association is reduced postexercise, apparently due to preferential utilization of PLIN2-associated LDs. These results confirm the hypothesis that the PLIN2 association with IMTG is related to the utilization of IMTG as a fuel during exercise.
Language eng
DOI 10.1113/expphysiol.2012.064592
Field of Research 111699 Medical Physiology not elsewhere classified
0606 Physiology
1116 Medical Physiology
1106 Human Movement And Sports Science
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089569

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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