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Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes

Shaw, C. S., Shepherd, S. O., Wagenmakers, A. J. M., Hansen, D., Dendale, P. and van Loon, L. J. C. 2012, Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes, American journal of physiology: endocrinology and metabolism, vol. 303, no. 9, pp. E1158-E1165, doi: 10.1152/ajpendo.00272.2012.

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Title Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes
Author(s) Shaw, C. S.ORCID iD for Shaw, C. S. orcid.org/0000-0003-1499-0220
Shepherd, S. O.
Wagenmakers, A. J. M.
Hansen, D.
Dendale, P.
van Loon, L. J. C.
Journal name American journal of physiology: endocrinology and metabolism
Volume number 303
Issue number 9
Start page E1158
End page E1165
Total pages 8
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2012-11-01
ISSN 1522-1555
Keyword(s) lipid metabolism
intramuscular triglyceride
adipocyte differentiation-related protein
adipophilin
insulin sensitivity
Abdominal Fat
Adiposity
Body Mass Index
Cohort Studies
Diabetes Mellitus, Type 2
Exercise
Humans
Insulin Resistance
Male
Membrane Proteins
Middle Aged
Mitochondria, Muscle
Muscle Fibers, Fast-Twitch
Muscle Fibers, Slow-Twitch
Overweight
Oxygen Consumption
Perilipin-2
Quadriceps Muscle
Time Factors
Triglycerides
Summary The aim of the present study was to investigate changes in intramuscular triglyceride (IMTG) content and perilipin 2 expression in skeletal muscle tissue following 6 mo of endurance-type exercise training in type 2 diabetes patients. Ten obese male type 2 diabetes patients (age 62 ± 1 yr, body mass index BMI 31 ± 1 kg/m2) completed three exercise sessions/week consisting of 40 min of continuous endurance-type exercise at 75% VO2 peak for a period of 6 mo. Muscle biopsies collected at baseline and after 2 and 6 mo of intervention were analyzed for IMTG content and perilipin 2 expression using fiber type-specific immunofluorescence microscopy. Endurance-type exercise training reduced trunk body fat by 6 ± 2% and increased whole body oxygen uptake capacity by 13 ± 7% (P < 0.05). IMTG content increased twofold in response to the 6 mo of exercise training in both type I and type II muscle fibers (P < 0.05). A threefold increase in perilipin 2 expression was observed from baseline to 2 and 6 mo of intervention in the type I muscle fibers only (1.1 ± 0.3, 3.4 ± 0.6, and 3.6 ± 0.6% of fibers stained, respectively, P < 0.05). Exercise training induced a 1.6-fold increase in mitochondrial content after 6 mo of training in both type I and type II muscle fibers (P < 0.05). In conclusion, this is the first study to report that prolonged endurance-type exercise training increases the expression of perilipin 2 alongside increases in IMTG content in a type I muscle fiber-type specific manner in type 2 diabetes patients.
Language eng
DOI 10.1152/ajpendo.00272.2012
Field of Research 110399 Clinical Sciences not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089572

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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