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Sprint interval and endurance training are equally effective in increasing muscle microvascular density and eNOS content in sedentary males

Cocks, Matthew, Shaw, Christopher S., Shepherd, Sam O., Fisher, James P., Ranasinghe, Aaron M., Barker, Thomas A., Tipton, Kevin D. and Wagenmakers, Anton J. M. 2013, Sprint interval and endurance training are equally effective in increasing muscle microvascular density and eNOS content in sedentary males, Journal of physiology, vol. 591, no. 3, pp. 641-656, doi: 10.1113/jphysiol.2012.239566.

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Title Sprint interval and endurance training are equally effective in increasing muscle microvascular density and eNOS content in sedentary males
Author(s) Cocks, Matthew
Shaw, Christopher S.
Shepherd, Sam O.
Fisher, James P.
Ranasinghe, Aaron M.
Barker, Thomas A.
Tipton, Kevin D.
Wagenmakers, Anton J. M.
Journal name Journal of physiology
Volume number 591
Issue number 3
Start page 641
End page 656
Total pages 16
Publisher Wileu
Place of publication London, Eng.
Publication date 2013-02
ISSN 1469-7793
Keyword(s) Adult
Bicycling
Blood Pressure
Glucose Tolerance Test
Humans
Insulin Resistance
Male
Membrane Glycoproteins
Microvessels
Muscle, Skeletal
NADPH Oxidase
Nitric Oxide Synthase Type III
Physical Endurance
Sedentary Lifestyle
Vascular Stiffness
Young Adult
Summary Sprint interval training (SIT) has been proposed as a time efficient alternative to endurance training (ET) for increasing skeletal muscle oxidative capacity and improving certain cardiovascular functions. In this study we sought to make the first comparisons of the structural and endothelial enzymatic changes in skeletal muscle microvessels in response to ET and SIT. Sixteen young sedentary males (age 21 ± SEM 0.7 years, BMI 23.8 ± SEM 0.7 kg m(-2)) were randomly assigned to 6 weeks of ET (40-60 min cycling at ∼65% , 5 times per week) or SIT (4-6 Wingate tests, 3 times per week). Muscle biopsies were taken from the m. vastus lateralis before and following 60 min cycling at 65% to measure muscle microvascular endothelial eNOS content, eNOS serine(1177) phosphorylation, NOX2 content and capillarisation using quantitative immunofluorescence microscopy. Whole body insulin sensitivity, arterial stiffness and blood pressure were also assessed. ET and SIT increased skeletal muscle microvascular eNOS content (ET 14%; P < 0.05, SIT 36%; P < 0.05), with a significantly greater increase observed following SIT (P < 0.05). Sixty minutes of moderate intensity exercise increased eNOS ser(1177) phosphorylation in all instances (P < 0.05), but basal and post-exercise eNOS ser(1177) phosphorylation was lower following both training modes. All microscopy measures of skeletal muscle capillarisation (P < 0.05) were increased with SIT or ET, while neither endothelial nor sarcolemmal NOX2 was changed. Both training modes reduced aortic stiffness and increased whole body insulin sensitivity (P < 0.05). In conclusion, in sedentary males SIT and ET are effective in improving muscle microvascular density and eNOS protein content.
Language eng
DOI 10.1113/jphysiol.2012.239566
Field of Research 110399 Clinical Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, The Authors and The Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089575

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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