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Paxillin and focal adhesion kinase colocalise in human skeletal muscle and its associated microvasculature

Wilson, Oliver J., Bradley, Helen, Shaw, Christopher S. and Wagenmakers, Anton J. M. 2014, Paxillin and focal adhesion kinase colocalise in human skeletal muscle and its associated microvasculature, Histochemistry and cell biology, vol. 142, no. 3, pp. 245-256, doi: 10.1007/s00418-014-1212-3.

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Title Paxillin and focal adhesion kinase colocalise in human skeletal muscle and its associated microvasculature
Author(s) Wilson, Oliver J.
Bradley, Helen
Shaw, Christopher S.ORCID iD for Shaw, Christopher S. orcid.org/0000-0003-1499-0220
Wagenmakers, Anton J. M.
Journal name Histochemistry and cell biology
Volume number 142
Issue number 3
Start page 245
End page 256
Total pages 12
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2014-09
ISSN 0948-6143
1432-119X
Keyword(s) focal adhesion
costamere
mechanotransduction
sarcolemma
endothelium
vascular smooth muscle
Adult
Fluorescent Antibody Technique
Focal Adhesion Kinase 1
Humans
Male
Microvessels
Muscle, Skeletal
Paxillin
Young Adult
Summary Focal adhesion kinase (FAK) and paxillin are functionally linked hormonal- and mechano-sensitive proteins. We aimed to describe paxillin's subcellular distribution using widefield and confocal immunofluorescence microscopy and test the hypothesis that FAK and paxillin colocalise in human skeletal muscle and its associated microvasculature. Percutaneous muscle biopsies were collected from the m. vastus lateralis of seven healthy males, and 5-μm cryosections were stained with anti-paxillin co-incubated with anti-dystrophin to identify the sarcolemma, anti-myosin heavy chain type I for fibre-type differentiation, anti-dihydropyridine receptor to identify T-tubules, lectin UEA-I to identify the endothelium of microvessels and anti-α-smooth muscle actin to identify vascular smooth muscle cells (VSMC). Colocalisation of anti-paxillin with anti-dystrophin or anti-FAK was quantified using Pearson's correlation coefficient on confocal microscopy images. Paxillin was primarily present in (sub)sarcolemmal regions of skeletal muscle fibres where it colocalised with dystrophin (r = 0.414 ± 0.026). The (sub)sarcolemmal paxillin immunofluorescence intensity was ~2.4-fold higher than in sarcoplasmic regions (P < 0.001) with sarcoplasmic paxillin immunofluorescence intensity ~10 % higher in type I than in type II fibres (P < 0.01). In some longitudinally orientated fibres, paxillin formed striations that corresponded to the I-band region. Paxillin immunostaining was highest in endothelial and VSMC and distributed heterogeneously in both cell types. FAK and paxillin colocalised at (sub)sarcolemmal regions and within the microvasculature (r = 0.367 ± 0.036). The first images of paxillin in human skeletal muscle suggest paxillin is present in (sub)sarcolemmal and I-band regions of muscle fibres and within the microvascular endothelium and VSMC. Colocalisation of FAK and paxillin supports their suggested role in hormonal and mechano-sensitive signalling.
Language eng
DOI 10.1007/s00418-014-1212-3
Field of Research 111699 Medical Physiology not elsewhere classified
1199 Other Medical And Health Sciences
Socio Economic Objective 920116 Skeletal System and Disorders (incl. Arthritis)
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30089578

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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