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Self assembly of HIV-1 Gag protein on lipid membranes generates PI(4,5)P₂/Cholesterol nanoclusters

Yandrapalli, Naresh, Lubart, Quentin, Tanwar, Hanumant S., Picart, Catherine, Mak, Johnson, Muriaux, Delphine and Favard, Cyril 2016, Self assembly of HIV-1 Gag protein on lipid membranes generates PI(4,5)P₂/Cholesterol nanoclusters, Scientific reports, vol. 6, Article number : 39332, pp. 1-13, doi: 10.1038/srep39332.

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Title Self assembly of HIV-1 Gag protein on lipid membranes generates PI(4,5)P₂/Cholesterol nanoclusters
Author(s) Yandrapalli, Naresh
Lubart, Quentin
Tanwar, Hanumant S.
Picart, Catherine
Mak, JohnsonORCID iD for Mak, Johnson orcid.org/0000-0002-5229-5707
Muriaux, Delphine
Favard, Cyril
Journal name Scientific reports
Volume number 6
Season Article number : 39332
Start page 1
End page 13
Total pages 13
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2016-12-23
ISSN 2045-2322
Keyword(s) Science & Technology
Summary The self-assembly of HIV-1 Gag polyprotein at the inner leaflet of the cell host plasma membrane is the key orchestrator of virus assembly. The binding between Gag and the plasma membrane is mediated by specific interaction of the Gag matrix domain and the PI(4,5)P2 lipid (PIP2). It is unknown whether this interaction could lead to local reorganization of the plasma membrane lipids. In this study, using model membranes, we examined the ability of Gag to segregate specific lipids upon self-assembly. We show for the first time that Gag self-assembly is responsible for the formation of PIP2 lipid nanoclusters, enriched in cholesterol but not in sphingomyelin. We also show that Gag mainly partition into liquid-disordered domains of these lipid membranes. Our work strongly suggests that, instead of targeting pre-existing plasma membrane lipid domains, Gag is more prone to generate PIP2/Cholesterol lipid nanodomains at the inner leaflet of the plasma membrane during early events of virus assembly.
Language eng
DOI 10.1038/srep39332
Field of Research 110804 Medical Virology
Socio Economic Objective 920109 Infectious Diseases
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID NHMRC 543107
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30090364

Document type: Journal Article
Collections: School of Medicine
Open Access Collection
Molecular and Medical Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.