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The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer)

Araújo, Bruno, Salini, Michael, Glencross, Brett and Wade, Nicholas 2016, The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer), Aquaculture research, vol. 48, no. 7, pp. 3879-3893, doi: 10.1111/are.13215.

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Title The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer)
Formatted title The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer)
Author(s) Araújo, Bruno
Salini, Michael
Glencross, Brett
Wade, Nicholas
Journal name Aquaculture research
Volume number 48
Issue number 7
Start page 3879
End page 3893
Total pages 15
Publisher Wiley
Place of publication London, Eng.
Publication date 2016-10-27
ISSN 1355-557X
1365-2109
Keyword(s) Asian sea bass
gene expression
liver
fatty acids,
LC-PUFA
Summary For many fish species, dietary fish oil (FO) has been substituted with other oils such as poultry oil (PO) without affecting growth performance. However, in barramundi, the mechanisms by which fatty acid metabolism is regulated are poorly understood, and the effects of FO substitution are unknown. This study defined changes in the expression of genes controlling the metabolism of fatty acids in barramundi over a 24-h time period after a single meal. From one to 12 h after a single feeding event, the expression of fatty acid synthesis genes in the liver was upregulated, while genes involved in the β-oxidation showed minimal alteration. However, the expression of β-oxidation genes was significantly correlated with the expression of genes regulating fatty acid synthesis. In a second experiment, the changes in liver fatty acid composition and gene expression were defined after FO was substituted with PO. Liver fatty acid profile reflected the diet composition, with some subtle exceptions supporting the enrichment of certain long-chain polyunsaturated fatty acids in the liver. The fish from all experimental groups preferentially retained more docosahexaenoic acid than eicosapentaenoic acid in the liver, suggesting a bioconversion of this fatty acid to intermediate fatty acids. Replacement of FO with PO significantly regulated genes controlling both fatty acid synthesis and catabolism pathways, potentially related to a higher percentage of monounsaturated fatty acids, in the livers of fish fed these diets. The results demonstrated that diet composition significantly altered the lipid metabolism in barramundi and that there was a balance between direct dietary effects and endogenous synthetic capacity.
Language eng
DOI 10.1111/are.13215
Field of Research 070401 Aquaculture
0704 Fisheries Sciences
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, John Wiley & Sons
Persistent URL http://hdl.handle.net/10536/DRO/DU:30090411

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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