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NMR profiling of transgenic peas

Charlton, Adrian, Allnutt, Theo, Holmes, Stephen, Chisholm, James, Bean, Samantha, Ellis, Noel, Mullineaux, Phil and Oehlschlager, Sarah 2004, NMR profiling of transgenic peas, Plant biotechnology journal, vol. 2, no. 1, pp. 27-35, doi: 10.1046/j.1467-7652.2003.00045.x.

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Title NMR profiling of transgenic peas
Author(s) Charlton, Adrian
Allnutt, Theo
Holmes, Stephen
Chisholm, James
Bean, Samantha
Ellis, Noel
Mullineaux, Phil
Oehlschlager, Sarah
Journal name Plant biotechnology journal
Volume number 2
Issue number 1
Start page 27
End page 35
Total pages 9
Publisher Wiley-Blackwell
Place of publication West Sussex, Eng.
Publication date 2004-01
ISSN 1467-7652
Keyword(s) fingerprinting
GMO
metabolite profiling
multivariate statistics
NMR spectroscopy
pea
Pisum sativum
transgenic
Summary A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. Multivariate analysis of 1H NMR (nuclear magnetic resonance) spectra obtained from uniformly grown glasshouse plants revealed differences between the transgenic and control group that exceeded the natural variation of the plants. When a larger data set of six related transgenic lines was analysed, including a null segregant in addition to the wild-type control, multivariate analysis showed that the distribution of metabolites in the transgenics was different from that of the null segregant. However, the profile obtained from the wild-type material was diverse in comparison with both the transgenics and the null segregant, suggesting that the primary cause of the observed differences was that the transformation process selects for a subset of individuals able to undergo the transformation and selection procedures, and that their descendants have a restricted variation in metabolite profile, rather than that the presence of the transgene itself generates these differences.
Language eng
DOI 10.1046/j.1467-7652.2003.00045.x
Field of Research 060499 Genetics not elsewhere classified
Socio Economic Objective 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Blackwell Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30090761

Document type: Journal Article
Collection: School of Medicine
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