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Coordination of cell cycle progression and mitotic spindle assembly involves histone H3 lysine 4 methylation by set1/COMPASS

Beilharz, Traude H, Harrison, Paul F, Miles, Douglas Maya, See, Michael Ming, Le, Uyen Minh Merry, Kalanon, Ming, Curtis, Melissa Jane, Hasan, Qambar, Saksouk, Julie, Margaritis, Thanasis, Holstege, Frank, Geli, Vincent and Dichtl, Bernhard 2017, Coordination of cell cycle progression and mitotic spindle assembly involves histone H3 lysine 4 methylation by set1/COMPASS, Genetics, vol. 205, no. 1, pp. 185-199, doi: 10.1534/genetics.116.194852.

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Title Coordination of cell cycle progression and mitotic spindle assembly involves histone H3 lysine 4 methylation by set1/COMPASS
Author(s) Beilharz, Traude H
Harrison, Paul F
Miles, Douglas Maya
See, Michael Ming
Le, Uyen Minh Merry
Kalanon, Ming
Curtis, Melissa Jane
Hasan, Qambar
Saksouk, Julie
Margaritis, Thanasis
Holstege, Frank
Geli, Vincent
Dichtl, Bernhard
Journal name Genetics
Volume number 205
Issue number 1
Start page 185
End page 199
Total pages 15
Publisher Genetics Society of America
Place of publication Austin, Tex.
Publication date 2017-01
ISSN 0016-6731
1943-2631
Keyword(s) aurora kinase
benomyl
cell cycle
gene expression
histone methylation
Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
Beta-tubulin gene
Saccharomyces-cerevisiae
Budding yeast
Alpha-tubulin
Transcriptional elongation
Tri-methylation
Bi-orientation
Coding regions
Active genes
Genome-wide
Summary Methylation of histone H3 lysine 4 (H3K4) by Set1 complex/COMPASS is a hallmark of eukaryotic chromatin, but it remains poorly understood how this post-translational modification contributes to the regulation of biological processes like the cell cycle. Here, we report a H3K4 methylation-dependent pathway in Saccharomyces cerevisiae that governs toxicity toward benomyl, a microtubule destabilizing drug. Benomyl-sensitive growth of wild-type cells required mono- and dimethylation of H3K4 and Pho23, a PHD-containing subunit of the Rpd3L complex. Δset1 and Δpho23 deletions suppressed defects associated with ipl1-2 aurora kinase mutant, an integral component of the spindle assembly checkpoint during mitosis. Benomyl resistance of Δset1 strains was accompanied by deregulation of all four tubulin genes and the phenotype was suppressed by tub2-423 and Δtub3 mutations, establishing a genetic link between H3K4 methylation and microtubule function. Most interestingly, sine wave fitting and clustering of transcript abundance time series in synchronized cells revealed a requirement for Set1 for proper cell-cycle-dependent gene expression and Δset1 cells displayed delayed entry into S phase. Disruption of G1/S regulation in Δmbp1 and Δswi4 transcription factor mutants duplicated both benomyl resistance and suppression of ipl1-2 as was observed with Δset1 Taken together our results support a role for H3K4 methylation in the coordination of cell-cycle progression and proper assembly of the mitotic spindle during mitosis.
Language eng
DOI 10.1534/genetics.116.194852
Field of Research 060499 Genetics not elsewhere classified
0604 Genetics
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, the Genetics Society of America
Persistent URL http://hdl.handle.net/10536/DRO/DU:30090773

Document type: Journal Article
Collections: Faculty of Health
Centre for Physical Activity and Nutrition Research
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