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A quantitative profiling method of phytohormones and other metabolites applied to barley roots subjected to salinity stress

Cao, Da, Lutz, Adrian, Hill, Camilla B., Callahan, Damien L. and Roessner, Ute 2017, A quantitative profiling method of phytohormones and other metabolites applied to barley roots subjected to salinity stress, Frontiers in plant science, vol. 7, pp. 1-19, doi: 10.3389/fpls.2016.02070.

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Title A quantitative profiling method of phytohormones and other metabolites applied to barley roots subjected to salinity stress
Author(s) Cao, Da
Lutz, Adrian
Hill, Camilla B.
Callahan, Damien L.ORCID iD for Callahan, Damien L. orcid.org/0000-0002-6384-8717
Roessner, Ute
Journal name Frontiers in plant science
Volume number 7
Article ID 2070
Start page 1
End page 19
Total pages 19
Publisher Frontiers Research Foundation
Place of publication Lausanne, Switzerland
Publication date 2017-01-10
ISSN 1664-462X
Keyword(s) phytohormone
liquid chromatography-mass spectrometry
gas chromatography-mass spectrometry
metabolomics
Na+ exclusion
salinity stress
barley root
hydroponics
Summary As integral parts of plant signaling networks, phytohormones are involved in the regulation of plant metabolism and growth under adverse environmental conditions, including salinity. Globally, salinity is one of the most severe abiotic stressors with an estimated 800 million hectares of arable land affected. Roots are the first plant organ to sense salinity in the soil, and are the initial site of sodium (Na + ) exposure. However, the quantification of phytohormones in roots is challenging, as they are often present at extremely low levels compared to other plant tissues. To overcome this challenge, we developed a high-throughput LC-MS method to quantify ten endogenous phytohormones and their metabolites of diverse chemical classes in roots of barley. This method was validated in a salinity stress experiment with six barley varieties grown hydroponically with and without salinity. In addition to phytohormones, we quantified 52 polar primary metabolites, including some phytohormone precursors, using established GC-MS and LC-MS methods. Phytohormone and metabolite data were correlated with physiological measurements including biomass, plant size and chlorophyll content. Root and leaf elemental analysis was performed to determine Na + exclusion and K + retention ability in the studied barley varieties. We identified distinct phytohormone and metabolite signatures as a response to salinity stress in different barley varieties. Abscisic acid increased in the roots of all varieties under salinity stress, and elevated root salicylic acid levels were associated with an increase in leaf chlorophyll content. Furthermore, the landrace Sahara maintained better growth, had lower Na + levels and maintained high levels of the salinity stress linked metabolite putrescine as well as the phytohormone metabolite cinnamic acid, which has been shown to increase putrescine concentrations in previous studies. This study highlights the importance of root phytohormones under salinity stress and the multi-variety analysis provides an important update to analytical methodology, and adds to the current knowledge of salinity stress responses in plants at the molecular level.
Language eng
DOI 10.3389/fpls.2016.02070
Field of Research 060705 Plant Physiology
060702 Plant Cell and Molecular Biology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30091072

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.