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Peroxide reduction by a metal-dependent catalase in Nostoc punctiforme (cyanobacteria)

Hudek, L., Torriero, A., Michalczyk, A., Neilan, B.A., Ackland, M. and Brau, L. 2017, Peroxide reduction by a metal-dependent catalase in Nostoc punctiforme (cyanobacteria), Applied microbiology and biotechnology, vol. 101, no. 9, pp. 3781-3800, doi: 10.1007/s00253-017-8130-y.

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Title Peroxide reduction by a metal-dependent catalase in Nostoc punctiforme (cyanobacteria)
Author(s) Hudek, L.ORCID iD for Hudek, L. orcid.org/0000-0002-5722-9346
Torriero, A.ORCID iD for Torriero, A. orcid.org/0000-0001-8616-365X
Michalczyk, A.ORCID iD for Michalczyk, A. orcid.org/0000-0001-5716-0783
Neilan, B.A.
Ackland, M.ORCID iD for Ackland, M. orcid.org/0000-0002-7474-6556
Brau, L.ORCID iD for Brau, L. orcid.org/0000-0001-7105-8339
Journal name Applied microbiology and biotechnology
Volume number 101
Issue number 9
Start page 3781
End page 3800
Total pages 20
Publisher Springer
Place of publication Berlin, Germany
Publication date 2017-05
ISSN 0175-7598
1432-0614
Keyword(s) reactive oxygen species (ROS)
catalase
peroxdidase
cyanobacteria
trace elements
Peroxidase
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
MULTIPLE SEQUENCE ALIGNMENT
ESCHERICHIA-COLI
OXIDATIVE STRESS
SUPEROXIDE-DISMUTASE
MEDICAGO-TRUNCATULA
ELECTRON-TRANSPORT
MANGANESE CATALASE
HYDROGEN-PEROXIDE
PCC 6803
ZINC
Summary This study investigated the role of a novel metal-dependent catalase (Npun_R4582) that reduces hydrogen peroxide in the cyanobacterium Nostoc punctiforme. Quantitative real-time PCR showed that npun_R4582 relative mRNA levels were upregulated by over 16-fold in cells treated with either 2 µM added Co, 0.5 µM added Cu, 500 µM Mn, 1 µM Ni or 18 µM Zn. For cells treated with 60 µM H2O2, no significant alteration in Npun_R4582 relative mRNA levels was detected, while in cells treated with Co, Cu, Mn, Ni or Zn and 60 µM peroxide, relative mRNA levels were generally above control or peroxide only treated cells. Disruption or overexpression of npun_R4582 altered sensitivity to cells exposed to 60 µM H2O2 and metals for treatments beyond the highest viable concentrations, or in a mixed metal solution for Npun_R4582- cells. Moreover, overexpression of npun_R4582 increased cellular peroxidase activity in comparison with wildtype and Npun_R4582- cells, and reduced peroxide levels by over 50%. The addition of cobalt, manganese, nickel and zinc increased the capacity of Npun_R4582 to reduce the rate or total levels of peroxide produced by cells growing under photooxidative conditions. The work presented confirms the function of NpunR4582 as a catalase and provides insights as to how cells reduce potentially lethal peroxide levels produced by photosynthesis. The findings also show how trace elements play crucial roles as enzymatic cofactors and how the role of Npun_R4582 in hydrogen peroxide breakdown is dependent on the type of metal and the level available to cells.
Language eng
DOI 10.1007/s00253-017-8130-y
Field of Research 060599 Microbiology not elsewhere classified
030599 Organic Chemistry not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30091073

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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