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Identification of rays through DNA barcoding: an application for ecologists

Cerutti-Pereyra, Florencia, Meekan, Mark G., Wei, Nu-Wei V., O'Shea, Owen, Bradshaw, Corey J. A. and Austin, Chris M. 2012, Identification of rays through DNA barcoding: an application for ecologists, PLoS One, vol. 7, no. 6, Article number : e36479, pp. 1-10, doi: 10.1371/journal.pone.0036479.

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Title Identification of rays through DNA barcoding: an application for ecologists
Author(s) Cerutti-Pereyra, Florencia
Meekan, Mark G.
Wei, Nu-Wei V.
O'Shea, Owen
Bradshaw, Corey J. A.
Austin, Chris M.
Journal name PLoS One
Volume number 7
Issue number 6
Season Article number : e36479
Start page 1
End page 10
Total pages 10
Publisher PLoS
Place of publication San Francisco, Calif.
Publication date 2012-06
ISSN 1932-6203
Keyword(s) Animals
Base Sequence
Bayes Theorem
Cluster Analysis
DNA Barcoding, Taxonomic
DNA Primers
Evolution, Molecular
Models, Genetic
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Skates (Fish)
Species Specificity
Western Australia
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FRESH-WATER CRAYFISH
SPOTTED EAGLE RAY
MITOCHONDRIAL-DNA
POPULATION-STRUCTURE
EVOLUTIONARY RELATIONSHIPS
SHARKS
AUSTRALIA
CONSERVATION
SEQUENCES
Summary DNA barcoding potentially offers scientists who are not expert taxonomists a powerful tool to support the accuracy of field studies involving taxa that are diverse and difficult to identify. The taxonomy of rays has received reasonable attention in Australia, although the fauna in remote locations such as Ningaloo Reef, Western Australia is poorly studied and the identification of some species in the field is problematic. Here, we report an application of DNA-barcoding to the identification of 16 species (from 10 genera) of tropical rays as part of an ecological study. Analysis of the dataset combined across all samples grouped sequences into clearly defined operational taxonomic units, with two conspicuous exceptions: the Neotrygon kuhlii species complex and the Aetobatus species complex. In the field, the group that presented the most difficulties for identification was the spotted whiptail rays, referred to as the 'uarnak' complex. Two sets of problems limited the successful application of DNA barcoding: (1) the presence of cryptic species, species complexes with unresolved taxonomic status and intra-specific geographical variation, and (2) insufficient numbers of entries in online databases that have been verified taxonomically, and the presence of lodged sequences in databases with inconsistent names. Nevertheless, we demonstrate the potential of the DNA barcoding approach to confirm field identifications and to highlight species complexes where taxonomic uncertainty might confound ecological data.
Language eng
DOI 10.1371/journal.pone.0036479
Field of Research 050199 Ecological Applications not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30091333

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.