Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling

Baranski, M., Rourke, M., Loughnan, S., Hayes, B, Austin, C. and Robinson, N. 2008, Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling, Animal genetics, vol. 39, no. 6, pp. 606-614, doi: 10.1111/j.1365-2052.2008.01773.x.

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Title Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling
Author(s) Baranski, M.
Rourke, M.
Loughnan, S.
Hayes, B
Austin, C.ORCID iD for Austin, C.
Robinson, N.
Journal name Animal genetics
Volume number 39
Issue number 6
Start page 606
End page 614
Total pages 9
Publisher Wiley-Blackwell
Place of publication London, Eng.
Publication date 2008-12
ISSN 0268-9146
Keyword(s) animals
genetic linkage
genetic markers
microsatellite repeats
polymerase chain reaction
quantitative trait loci
growth rate
Haliotis rubra
selective DNA pooling
Summary The objective of this study was to identify QTL for growth rate in the blacklip abalone Haliotis rubra using selective DNA pooling. Three full-sibling families of H. rubra derived from crosses of wild broodstock were used. DNA was extracted from the largest and smallest 10% of progeny and combined into two pools for each phenotypic tail. The DNA pools were typed with 139 microsatellites, and markers showing significant differences between the peak height ratios of alleles inherited from the parents were individually genotyped and analysed by interval mapping. A strong correlation (r = 0.94, P < 0.001) was found between the t-values from the analysis of pools and the t-values from the analysis of individual genotypes. Based on the interval mapping analysis, QTL were detected on nine linkage groups at a chromosome-wide P < 0.01 and one linkage group at a chromosome-wide P < 0.05. The study demonstrated that selective DNA pooling is efficient and effective as a first-pass screen for the discovery of QTL in an aquaculture species.
Language eng
DOI 10.1111/j.1365-2052.2008.01773.x
Field of Research 060408 Genomics
0707 Veterinary Sciences
0608 Zoology
0604 Genetics
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2008, The Authors, journal complilation & INternational Society for Animal Genetics
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