Controlled assembly of silver nano-fluid in Heliotropium crispum extract: a potent anti-biofilm and bactericidal formulation

Khan, Faria, Hashmi, Muhammad Uzair, Khalid, Nauman, Hayat, Muhammad Qasim, Ikram, Aamer and Janjua, Hussnain A. 2016, Controlled assembly of silver nano-fluid in Heliotropium crispum extract: a potent anti-biofilm and bactericidal formulation, Applied surface science, vol. 387, pp. 317-331, doi: 10.1016/j.apsusc.2016.05.133.

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Title Controlled assembly of silver nano-fluid in Heliotropium crispum extract: a potent anti-biofilm and bactericidal formulation
Author(s) Khan, Faria
Hashmi, Muhammad Uzair
Khalid, NaumanORCID iD for Khalid, Nauman
Hayat, Muhammad Qasim
Ikram, Aamer
Janjua, Hussnain A.
Journal name Applied surface science
Volume number 387
Start page 317
End page 331
Total pages 15
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-11-30
ISSN 0169-4332
Keyword(s) silver nanoparticles
anti-biofilm agents
Heliotropium crispum
bactericidal effect
green synthesis
Summary The study describes the optimized method for silver nanoparticle (AgNPs) synthesis using Heliotropium crispum (HC) plant extract. Optimization of physicochemical parameters resulted in stable and rapidly assembled AgNPs. FTIR results suggest presence of plant phytochemicals that helped in the reduction, stabilization and capping of AgNPs. The assembled Ag nano-composites displayed the peak surface plasmon resonance (SPR) around 428 nm. The presence of uniquely assembled Ag-biomolecule composites, cap and stabilize nanoparticles in aqueous plant suspension. Spherical, uniform-shaped AgNPs with low poly-dispersion and average particle size of 42 nm and was determined through dynamic light scattering (DLS) and scanning election microscopy (SEM) which present robust interaction with microbes. The study also evaluates the antimicrobial and anti-biofilm properties of biologically synthesized AgNPs on clinical isolates of MRSA, Pseudomonas aeruginosa and Acinetobacter baumannii. Minimum inhibitory concentration (0.5 mg mL−1) of nanoparticles that presented bactericidal effect was made through inhibition assays on bacterial strains. The concentration which presented potent bactericidal response was then evaluated through growth inhibition in liquid medium for anti-biofilm studies at 2.0 mg mL−1. HC-Ag nanoparticles mediated anti-biofilm effects on Pseudomonas aeruginosa was revealed through SEM. Complete breakdown of biofilm's extracellular polymeric substances resulted after incubation with AgNPs. Peptidoglycan cell wall destruction was also revealed on planktonic bacterial images after 24 h of incubation.
Language eng
DOI 10.1016/j.apsusc.2016.05.133
Field of Research 060599 Microbiology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
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